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AB250252

Anti-RNF214 antibody [EPR12940] - BSA and Azide free

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Rabbit Recombinant Monoclonal RNF214 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

RING finger protein 214, RNF214

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)

This data was developed using ab180850, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells labeling RNF214 using ab180850 at 1/50 dilution.

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)
  • IP

Supplier Data

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)

This data was developed using ab180850, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HT-1376 cell lysate labeling RBF214 using ab180850 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (<a href='/ko/products/primary-antibodies/rnf214-antibody-epr12940-ab180850'>ab180850</a>)

Predicted band size: 78 kDa

false

Western blot - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)
  • WB

Supplier Data

Western blot - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (AB250252)

This data was developed using ab180850, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF214 antibody [EPR12940] (<a href='/ko/products/primary-antibodies/rnf214-antibody-epr12940-ab180850'>ab180850</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

HT-1376 lysates at 10 µg

Lane 3:

HepG2 lysates at 10 µg

Predicted band size: 78 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12940

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB129002

Anti-Vinculin antibody [EPR8185] - Loading Control

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - Loading Control (AB129002)

  • 5
  • 21
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB182560

Anti-ARID1A antibody [EPR13501]

Recombinant|KO Validated|Advanced Validation|RabMAb|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [EPR13501] (AB182560)

  • 4
  • 1
Primary Antibodies

AB181131

Anti-PTGR1 antibody [EPR13451-10]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTGR1 antibody [EPR13451-10] (AB181131)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

ab250252 is the carrier-free version of ab180850.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RNF214 also known as Ring Finger Protein 214 is an E3 ubiquitin-protein ligase. It plays a role in tagging proteins with ubiquitin for regulated protein degradation. The protein has a mass of approximately 74 kDa. RNF214 shows expression in many tissues including prostate brain and ovary. This wide expression pattern implies a diverse function potential in cellular processes.
Biological function summary

RNF214 participates in protein homeostasis by facilitating the ubiquitination process. It functions as part of an E3 ligase complex which delivers ubiquitin molecules to substrate proteins. This modification signals these proteins for degradation by the proteasome. Through this activity RNF214 influences protein turnover and maintains cell homeostasis by removing damaged or misfolded proteins ensuring functional protein networks.

Pathways

RNF214 is involved in the ubiquitin-proteasome system and the regulation of MAPK signaling pathways. The protein works closely with other ubiquitin ligases to control protein levels impacting cellular responses like cell cycle and growth. In the MAPK signaling pathway RNF214 can modulate the stability of signaling molecules like MAPK which impacts cell proliferation and differentiation.

RNF214 has associations with neurodegenerative diseases and cancer. Aberrant functioning of RNF214 could lead to an accumulation of misfolded proteins contributing to neurodegenerative conditions like Alzheimer's disease. In cancer changes in RNF214 activity might disrupt normal cell cycle control leading to tumorigenesis. It often connects with other proteins like p53 in the context of these diseases highlighting the importance of its regulatory role in cellular homeostasis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information RNF214
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang
제품이 사용된 논문 모두 보기
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Product promise

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