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AB236992

Anti-RNF220 antibody

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Rabbit Polyclonal RNF220 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RNF220 aa 350-500.

대체 명칭 보기

C1orf164, RNF220, E3 ubiquitin-protein ligase RNF220, RING finger protein 220, RING-type E3 ubiquitin transferase RNF220

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF220 antibody (AB236992)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF220 antibody (AB236992)

Paraffin-embedded human prostate cancer tissue stained for RNF220 using ab236992 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4° overnight. The primary is detected by a biotinylated secondary and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RNF220 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q5VTB9

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대체품

Primary Antibodies

AB69357

Anti-RNF220 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RNF220 antibody (AB69357)

  • 0
  • 0

보완 제품

Multiplex Kits

AB197484

Rat Inflammation Antibody Array (10 Targets) - Quantitative

Multiplex Protein Detection - Rat Inflammation Antibody Array (10 Targets) - Quantitative (AB197484)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
  • 90
Primary Antibodies

AB311130

Alexa Fluor® 647 Anti-PHOX2B antibody [EPR14423] - C-terminal

RabMAb|Recombinant

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RNF220 also known as Ring Finger Protein 220 is an E3 ubiquitin-protein ligase. It has a molecular weight of approximately 45 kDa. As a member of the RING finger protein family RNF220 participates in the process of attaching ubiquitin molecules to target proteins which usually marks them for degradation by the proteasome. This protein is broadly expressed in human tissues with significant expression in the testis ovaries and brain.
Biological function summary

RNF220 plays a role in the regulation of transcription factors and is part of certain protein complexes. It interacts with the ubiquitination machinery to control the stability and activity of various transcriptional regulators influencing gene expression patterns essential for cellular differentiation and development. This protein contributes to the regulation of right/left asymmetry in embryonic development through its interaction with key developmental transcription factors.

Pathways

RNF220 plays a role in the Wnt signaling pathway and the Hedgehog signaling pathway. Through these pathways it influences cellular proliferation and differentiation. It interacts with proteins such as β-catenin in the Wnt pathway and GLI proteins in the Hedgehog pathway. These interactions regulate the transcriptional output of these signaling cascades influencing processes like embryogenesis and tissue homeostasis.

RNF220 links to developmental disorders and certain cancers. Mutations or dysregulation of RNF220 can contribute to conditions such as congenital asymmetry disorders through its interaction with transcription factors like Pitx2. Additionally its role in the Wnt and Hedgehog pathways implicates it in cancers including medulloblastoma where it interacts with proteins involved in regulating cell cycle and apoptosis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (By similarity). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling (PubMed : 25266658, PubMed : 33964137). Plays a critical role in the regulation of nuclear lamina (PubMed : 33964137).
See full target information RNF220
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 9:eadj4198 PubMed37862421

2023

Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoyuki Shiota,Zhucui Li,Guan-Yuan Chen,Kevin L McKnight,Takayoshi Shirasaki,Bryan Yonish,Heyjeong Kim,Ethan J Fritch,Timothy P Sheahan,Masamichi Muramatsu,Maryna Kapustina,Craig E Cameron,You Li,Qibin Zhang,Stanley M Lemon
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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