Anti-ROCK2 (phospho S1366) antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded human breast tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROCK2 (phospho S1366) (green) using ab228008 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded HBL435 xenograft tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded mouse brain tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (AB228008)

Paraffin-embedded rat kidney tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lanes 2 - 3:

ROCK2 shRNA transfected HEK-293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 161 kDa

false

• WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody (AB228008)

HEK-293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCK2 expression constructs. Overexpressed Myc-ROCK2 proteins were immunoprecipitated with anti-myc antibody and probed with ab228008 with or without peptide (0.2 mg/ml) competition as indicated.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/1000 dilution

Lanes 1, 3 and 5:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with WT ROCK2, whole cell lysate

Lanes 2, 4 and 6:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with phospho-mutant (S1366A) ROCK2, whole cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 161 kDa

false