AB182648

Anti-ROCK2 (phospho Y722) antibody

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(6 제품이 사용된 논문 )

Rabbit Polyclonal ROCK2 phospho Y722 antibody. Suitable for WB and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ROCK2 pY722 conjugated to Keyhole Limpet Haemocyanin.

1 이미지

Supplier Data

Western blot - Anti-ROCK2 (phospho Y722) antibody (AB182648)

All lanes:

Western blot - Anti-ROCK2 (phospho Y722) antibody (ab182648) at 1/500 dilution

Lane 1:

Rat kidney extract with antigen-specific peptide

Lane 2:

Rat kidney extract

Predicted band size: 161 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human ROCK2 pY722 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O75116

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab182648 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

See full target information ROCK2 pY722

대체 명칭 보기

KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록and refine your search

Molecules (Basel, Switzerland) 27: PubMed35889443

2022

Protection against Hypoxia-Reoxygenation Injury of Hippocampal Neurons by HS via Promoting Phosphorylation of ROCK at Tyr722 in Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Xue,Shuo Chen,Jiaojiao Xi,Qianjun Guan,Wei Chen,Yan Guo,Zhiwu Chen

Cell death & disease 13:138 PubMed35145081

2022

Acquired temozolomide resistance in MGMT gliomas is associated with regulation of homologous recombination repair by ROCK2.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhang,Tao Li,Mengdi Yang,Qianming Du,Rui Wang,Bin Fu,Yingying Tan,Mengran Cao,Yaxin Chen,Qing Wang,Rong Hu

Annals of translational medicine 9:1543 PubMed34790749

2021

MYO1B enhances colorectal cancer metastasis by promoting the F-actin rearrangement and focal adhesion assembly via RhoA/ROCK/FAK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lang Xie,Hongyun Huang,Zheng Zheng,Qian Yang,Shubo Wang,Yaoxu Chen,Jinlong Yu,Chunhui Cui

Cell death & disease 9:190 PubMed29416017

2018

Fasudil increases temozolomide sensitivity and suppresses temozolomide-resistant glioma growth via inhibiting ROCK2/ABCG2.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhang,Xiuting Liu,Wei Zhou,Mengdi Yang,Yang Ding,Qing Wang,Rong Hu

Cell discovery 3:17044 PubMed29238610

2017

A role for ErbB signaling in the induction of reactive astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Wanwan He,Xu Hu,Yuwen Shen,Junyan Cao,Zhengdong Wei,Yifei Luan,Li He,Fangdun Jiang,Yanmei Tao

Oncotarget 8:104104-104112 PubMed29262624

2017

Fasudil attenuates soluble fms-like tyrosine kinase-1 (sFlt-1)-induced hypertension in pregnant mice through RhoA/ROCK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Gu,Yaling Feng,Jinjin Yu,Hua Yuan,Yongxiang Yin,Jian Ding,Jun Zhao,Yaohui Xu,Jianjuan Xu,Haisha Che

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ROCK2 (phospho Y722) antibody

Western blot - Anti-ROCK2 (phospho Y722) antibody (AB182648)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise