AB236409

Anti-ROMO1 antibody [OTI2C12]

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(4 제품이 사용된 논문 )

Mouse Monoclonal ROMO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Human, Mouse, Dog, African green monkey, Human, Transfected cell line - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ROMO1.

대체 명칭 보기

C20orf52, ROMO1, Reactive oxygen species modulator 1, ROS modulator 1, Epididymis tissue protein Li 175, Glyrichin, Mitochondrial targeting GxxxG motif protein, Protein MGR2 homolog, MTGM

12 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human thyroid tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human pancreas tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human endometrial adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human colon adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human kidney tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human breast adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human prostate tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human ovary adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

All lanes:

Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

SV-T2 (Mouse cell line) whole cell lysate at 10 µg

Lane 4:

A549 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 10 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 7:

MDCK (canine kidney cell line) whole cell lysate at 10 µg

Predicted band size: 8 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ROMO1 stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.

Supplier Data

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

All lanes:

Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate at 5 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ROMO1, whole cell lysate at 5 µg

Predicted band size: 8 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2C12

Isotype

IgG1

Carrier free

Reacts with

Mouse, Dog, Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ROMO1.

P60602

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Clone OTI2C12 (formerly 2C12)

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation.. Has antibacterial activity against a variety of bacteria including S.aureus, P.aeruginosa and M.tuberculosis. Acts by inducing bacterial membrane breakage.

See full target information ROMO1

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Animal bioscience 38:303-315 PubMed39210798

2024

shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Cheng,Shu Zhen Jiang,Li Bo Huang,Wei Ren Yang

Toxins 14: PubMed36422967

2022

Zearalenone Exposure Affects the Keap1-Nrf2 Signaling Pathway and Glucose Nutrient Absorption Related Genes of Porcine Jejunal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Cheng,Shuzhen Jiang,Libo Huang,Yuxi Wang,Weiren Yang

Journal of digestive diseases 23:376-387 PubMed35844201

2022

Circular RNA circ_0006089 promotes the progression of gastric cancer by regulating the miR-143-3p/PTBP3 axis and PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Rong Lin,Wei Rong Chen,Pei Hong Zheng,Wei Shan Chen,Gao Yang Cai

Cellular and molecular neurobiology 42:2379-2392 PubMed34089427

2021

GPR18 Agonist Resolvin D2 Reduces Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage by Multiple Protective Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tongyu Zhang,Gang Zuo,Hongqi Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ROMO1 antibody [OTI2C12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise