AB254898

Anti-RPL18 antibody

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Rabbit Polyclonal RPL18 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPL18 aa 50-200.

대체 명칭 보기

Large ribosomal subunit protein eL18, 60S ribosomal protein L18, RPL18

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RPL18 antibody (AB254898)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL18 antibody (AB254898)

PFA fixed, Triton X-100 permeabilized HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling RPL18 using ab254898 at 4μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL18 antibody (AB254898)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL18 antibody (AB254898)

Paraffin-embedded human pancreas tissue stained for RPL18 using ab254898 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-RPL18 antibody (AB254898)

Lane 1:

Western blot - Anti-RPL18 antibody (ab254898)

Lanes 2 - 3:

Western blot - Anti-RPL18 antibody (ab254898) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 3:

U-251MG sp whole cell lysate

Predicted band size: 22 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RPL18 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q07020

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL18 also known as Ribosomal Protein L18 is a component of the 60S large ribosomal subunit. It possesses a molecular weight of approximately 22 kDa. RPL18 is highly expressed in various tissues including the liver brain and pancreas. The protein plays an important role in protein synthesis by aiding in the assembly and function of ribosomes essential cellular machinery for translating mRNA into polypeptides.

Biological function summary

RPL18 functions as part of the ribosomal complex facilitating mRNA translation into proteins. This protein is integral to the structure of the ribosome which serves as the platform for accurate peptide bond formation between amino acids. RPL18 like other ribosomal proteins contributes to the stability and efficiency of the ribosome's function during the translation process. Its presence ensures the proper alignment and interaction of ribosomal RNA and other ribosomal proteins.

Pathways

RPL18 is involved in key processes like the protein synthesis pathway and the ribosomal biogenesis pathway. In these pathways RPL18 interacts closely with other ribosomal proteins such as RPL5 and RPL11 which together form the core of the ribosomal assembly machinery. It also plays a role in the regulation of p53 through its interaction with these and other ribosomal proteins that influence cellular stress responses and cell cycle arrest.

Alterations in RPL18 levels or function can be linked to cancer and Diamond-Blackfan anemia. In cancer biology changes in ribosomal proteins including RPL18 may lead to disruptions in the normal patterns of protein synthesis contributing to tumorigenesis. In Diamond-Blackfan anemia a disorder characterized by bone marrow failure mutations in ribosomal proteins like RPL18 can impair ribosome function and affect hematopoiesis. RPL18's association with these conditions often involves interactions with key proteins such as p53 highlighting its broader impact on disease mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547).

See full target information RPL18

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research : CR 40:134 PubMed33858489

2021

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junshuang Zhao,Yang Jiang,Haiying Zhang,Jinpeng Zhou,Lian Chen,Hao Li,Jinkun Xu,Guoqing Zhang,Zhitao Jing

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPL18 antibody (AB254898)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL18 antibody (AB254898)

Western blot - Anti-RPL18 antibody (AB254898)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise