AB241418

Anti-RPL18A antibody

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Rabbit Polyclonal RPL18A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPL18A aa 100 to C-terminus.

대체 명칭 보기

Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, RPL18A

2 이미지

Supplier Data

Immunoprecipitation - Anti-RPL18A antibody (AB241418)

RPL18A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241418 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241418 at 0.4 μg/ml.

Lane 1 : ab241418 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RPL18A antibody (ab241418)

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-RPL18A antibody (AB241418)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RPL18A antibody (ab241418) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 21 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPL18A aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02543

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to RPL18A immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL18A also known as 60S ribosomal protein L18a plays a part in ribosome structure and function. This ribosomal protein with a molecular mass of approximately 22 kDa is an important part of the large 60S subunit of the ribosome. It is present in various tissues and has ubiquitous expression reflecting its role in protein synthesis. It functions as a structural constituent and it interacts directly with ribosomal RNA to help create the core framework necessary for ribosome stability.

Biological function summary

Ribosomal protein L18a contributes to the assembly and function of ribosomes the essential machines for translation of mRNA into proteins. It is a part of the ribosomal complex which includes numerous proteins and RNA molecules that collaborate for translation. L18a assists in maintaining the proper structure needed for the translation process impacting cell growth and proliferation. Also ribosomal proteins like RPL18A have non-ribosomal roles in regulating cell cycle and apoptosis showing their importance beyond protein synthesis.

Pathways

Ribosome biogenesis and protein synthesis involve RPL18A. It is a component of the ribosomal pathway closely related to other ribosomal proteins like RPL5 and RPL11 essential for the synthesis of new proteins. RPL18A also intersects with the mTOR signaling pathway which senses nutritional and energy conditions to regulate cell growth emphasizing its role in metabolic control.

Mutations or dysregulation of RPL18A connect to conditions such as Diamond-Blackfan anemia a rare genetic disorder resulting in failure to make red blood cells. RPL18A mutation leads to disrupted ribosome biogenesis similarly observed in many cancers where rapid cell growth demands increased protein synthesis. The interaction between RPL18A and other ribosomal proteins like RPL11 suggests their combined influence on the progression of these disorders by altering ribosomal function and cellular homeostasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL18A

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL18A antibody

Immunoprecipitation - Anti-RPL18A antibody (AB241418)

Western blot - Anti-RPL18A antibody (AB241418)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise