AB241088

Anti-RPL23 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal RPL23 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPL23 aa 50 to C-terminus.

대체 명칭 보기

Large ribosomal subunit protein uL14, 60S ribosomal protein L17, 60S ribosomal protein L23, RPL23

2 이미지

Supplier Data

Immunoprecipitation - Anti-RPL23 antibody (AB241088)

RPL23 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction, 20% of IP loaded, prepared using NETN lysis buffer) using ab241088 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab241088 at 1 μg/mL.

Lane 1 : ab241088 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-RPL23 antibody (ab241088)

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-RPL23 antibody (AB241088)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPL23 antibody (ab241088) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 15 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPL23 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62829

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL23 also known as ribosomal protein L23 is an essential component of the large 60S ribosomal subunit with a molecular mass of approximately 15 kDa. This protein is highly expressed in eukaryotic cells particularly in tissues with high rates of protein synthesis. RPL23 interacts directly with rRNA to facilitate its structural stability and function playing an important role in the assembly of the ribosome and the translation of mRNA into proteins.

Biological function summary

RPL23 contributes to the synthesis of proteins by forming part of the ribosomal complex. Ribosomes are composed of two subunits 40S and 60S in eukaryotes with RPL23 being integral to the larger subunit. The presence of RPL23 ensures that the ribosomes effectively translate the genetic code carried by mRNA into a polypeptide chain. This protein is essential in maintaining the fidelity and efficiency of translation processes within cells determining how cells grow and respond to various stimuli.

Pathways

RPL23 plays a significant role in the translation initiation pathway and the protein synthesis pathway which are important for cell growth and replication. Within these pathways RPL23 associates with other ribosomal proteins such as RPL5 and RPL11 which together modulate the formation of the ribosomal structure and oversee the translation process. The function of RPL23 in these pathways ensures that cellular resources are properly managed and directed towards productive protein synthesis.

Mutations or dysregulation of RPL23 have connections to cancer and Diamond-Blackfan anemia. The alteration in RPL23 can disrupt normal ribosome function leading to impaired protein synthesis and contributing to the uncontrolled cell growth seen in cancers. This protein also interacts with tumor suppressor proteins like TP53 linking its activity to mechanisms that control cell cycle and apoptosis. Furthermore deficiencies in RPL23 function may lead to insufficient production of red blood cells evident in Diamond-Blackfan anemia highlighting a relationship with genes responsible for balanced erythropoiesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL23

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

NAR cancer 7:zcaf024 PubMed40918646

2025

Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Dopler,Edwin S Kyei-Baffour,Mandy Kerkhoff,Ferhat Alkan,Yuval Malka,Kelly Hoefakker,Rob van der Kammen,Liesbeth Hoekman,Onno Bleijerveld,Antonia Bradaric,Maarten Altelaar,Jonathan W Yewdell,Pia Kvistborg,William J Faller

American journal of medical genetics. Part A 191:2757-2767 PubMed37596828

2023

Novel association of Dandy-Walker malformation with CAPN15 variants expands the phenotype of oculogastrointestinal neurodevelopmental syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

M Makenzie Beaman,Lucia Guidugli,Monia Hammer,Chelsea Barrows,Anne Gregor,Sangmoon Lee,Kristen L Deak,Marie T McDonald,Courtney Jensen,Maha S Zaki,Amira T Masri,Charlotte A Hobbs,Joseph G Gleeson,Jennifer L Cohen

Oncology reports 43:1863-1874 PubMed32236588

2020

Triptolide interrupts rRNA synthesis and induces the RPL23‑MDM2‑p53 pathway to repress lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wang,Zhi-Qian Zhang,Fang-Qiong Li,Jia-Ning Chen,Xiaoting Gong,Bei-Bei Cao,Wei Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL23 antibody

Immunoprecipitation - Anti-RPL23 antibody (AB241088)

Western blot - Anti-RPL23 antibody (AB241088)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise