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AB223089

Anti-RPL23A antibody

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Rabbit Polyclonal RPL23A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L23a aa 1-100.

대체 명칭 보기

Large ribosomal subunit protein uL23, 60S ribosomal protein L23a, RPL23A

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-RPL23A antibody (AB223089)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL23A antibody (AB223089)

A549 (human lung carcinoma cell line) cells stained for RPL23A (green) using ab223089 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL23A antibody (AB223089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL23A antibody (AB223089)

Paraffin-embedded human testis tissue stained for RPL23A using ab223089 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human 60S ribosomal protein L23a aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P62750

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대체품

Primary Antibodies

AB157110

Anti-RPL23A antibody

Immunoprecipitation - Anti-RPL23A antibody (AB157110)

  • 0
  • 0

보완 제품

Primary Antibodies

AB210745

Anti-RPS5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPS5 antibody (AB210745)

  • 0
  • 0
Primary Antibodies

AB196652

Anti-RPS24 antibody [EPR16017(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

  • 0
  • 0
Primary Antibodies

AB59567

Anti-RPL26 + RPL26L1 antibody

Western blot - Anti-RPL26 + RPL26L1 antibody (AB59567)

  • 3
  • 3
Primary Antibodies

AB88514

Anti-RPL29 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL29 antibody (AB88514)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL23A also known as Ribosomal Protein L23a is a component of the 60S subunit in the ribosome which is responsible for protein synthesis. It has a molecular mass of approximately 15 kDa. RPL23A is expressed in various tissues but shows significant expression levels in the liver and pancreas. The protein plays a mechanical role by ensuring structural stability and proper functioning of the ribosome during translation.
Biological function summary

RPL23A participates in the assembly and function of the ribosomal complex critical for translation. Within this complex RPL23A contributes to the coordination of protein synthesis by helping to align mRNA and tRNA correctly. This alignment is essential for the catalysis of peptide bond formation between amino acids allowing for the accurate synthesis of proteins.

Pathways

RPL23A is integral to the mTOR signaling pathway and the p53 signaling pathway. The mTOR pathway regulates cell growth proliferation and survival in response to nutrients growth factors and cellular energy levels with RPL23A interacting with proteins like TSC1 and TSC2. In the p53 signaling pathway RPL23A can influence cellular responses to DNA damage and stress by interacting with proteins such as MDM2.

RPL23A is implicated in cancer and Diamond-Blackfan anemia. In cancer the dysregulation of RPL23A can affect ribosome biogenesis and cell proliferation often seen in conjunction with increased levels of proteins like c-Myc. In Diamond-Blackfan anemia mutations or malfunctions in RPL23A lead to defective ribosomal function impacting erythroid progenitor cells and causing anemia with links to other ribosomal proteins such as RPS19.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547). Binds a specific region on the 26S rRNA (PubMed : 23636399, PubMed : 32669547). May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination (PubMed : 26203195).
See full target information 60S ribosomal protein L23a
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cell science 136: PubMed37232246

2023

Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families.

Applications

Unspecified application

Species

Unspecified reactive species

Beverley Wilson,Chloe Flett,Jakub Gemperle,Craig Lawless,Matthew Hartshorn,Eleanor Hinde,Tess Harrison,Megan Chastney,Sarah Taylor,Jennifer Allen,Jim C Norman,Thomas Zacharchenko,Patrick T Caswell
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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