AB241409

Anti-RPL27/RPL27A antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal RPL27/RPL27A antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPL27 aa 50 to C-terminus.

대체 명칭 보기

Large ribosomal subunit protein eL27, 60S ribosomal protein L27, RPL27

1 이미지

Supplier Data

Western blot - Anti-RPL27/RPL27A antibody (AB241409)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPL27/RPL27A antibody (ab241409) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 16 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human RPL27 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61353

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to RPL27/RPL27A immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL27 and RPL27A also known as ribosomal protein L27 and L27A are components of the large ribosomal subunit and weigh approximately 16 kDa. They function mechanically as integral parts of the ribosome which is important for protein synthesis. These proteins occur widely in the cytoplasm of eukaryotic cells contributing to the assembly of ribosomes which are vital for translating mRNA into polypeptides.

Biological function summary

RPL27 and RPL27A function as essential components of the ribosomal machinery that drives protein translation. Both proteins contribute to ribosome biogenesis an important process in cellular protein synthesis. As part of the large 60S ribosomal subunit they participate in the formation and stabilization of the ribosomal structure enabling efficient mRNA translation. These ribosomal proteins work within a complex that includes other ribosomal proteins and ribosomal RNA (rRNA) to facilitate the synthesis of proteins necessary for cell growth and function.

Pathways

RPL27 and RPL27A play significant roles in the translation pathway an essential part of gene expression. They are involved in the initiation elongation and termination phases of protein synthesis. The function of these proteins parallels that of other ribosomal proteins like RPL23 which helps in joining amino acids to form polypeptides. Additionally RPL27 and RPL27A integrate into pathways managing cellular responses to stress by regulating the production of proteins under different conditions such as during nutrient availability or cell proliferation signaling.

Alterations in RPL27 and RPL27A levels or mutations can associate with conditions like Diamond-Blackfan anemia and certain cancers. These proteins have links to oncogenesis as irregularities in ribosome biogenesis can lead to abnormal cell growth and cancer development. In addition their interaction with other ribosomal proteins such as RPL5 in these contexts indicates a broader impact on cellular homeostasis and susceptibility to disease. Understanding the role of these proteins in disease may provide insights into novel therapeutic approaches.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547). Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs (PubMed : 25424902).

See full target information RPL27

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine reports 28: PubMed37326104

2023

Construction of ceRNA regulatory networks for osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtao Chen,Hailong Wang,Xu Liu,Long Li,Yiliyaer Abudusimu,Yilihamu Tuoheti

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL27/RPL27A antibody

Western blot - Anti-RPL27/RPL27A antibody (AB241409)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise