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AB241414

Anti-RPL30 antibody

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Rabbit Polyclonal RPL30 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human 60S ribosomal protein L30 aa 1-50.

대체 명칭 보기

Large ribosomal subunit protein eL30, 60S ribosomal protein L30, RPL30

1 이미지
Western blot - Anti-RPL30 antibody (AB241414)
  • WB

Supplier Data

Western blot - Anti-RPL30 antibody (AB241414)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPL30 antibody (ab241414) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 13 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human 60S ribosomal protein L30 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P62888

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대체품

Reagents, Controls & Accessories

AB269262

RPL30 positive control ChIP primer pair

ChIP - RPL30 positive control ChIP primer pair (AB269262)

  • 0
  • 0
Primary Antibodies

AB170930

Anti-RPL30 antibody [EPR11624]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPL30 antibody [EPR11624] (AB170930)

  • 0
  • 0

보완 제품

Primary Antibodies

AB264369

Anti-RPL23 antibody

Immunoprecipitation - Anti-RPL23 antibody (AB264369)

  • 0
  • 0
Primary Antibodies

AB234792

Anti-RPL22L1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL22L1 antibody (AB234792)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB151550

Anti-RPS10 antibody [EPR8545]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab241414 was affinity purified using an epitope specific to RPL30 immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL30 also known as 60S ribosomal protein L30 is a component of the large 60S subunit of the ribosome. It has a molecular mass of approximately 12 kDa and is commonly expressed in all cells due to its essential role in protein synthesis. RPL30 functions mechanically by interacting with ribosomal RNA (rRNA) and other ribosomal proteins to form the ribosome structure ensuring proper translation of mRNA into proteins.
Biological function summary

Ribosomal protein L30 participates in the assembly of the ribosome complex which is responsible for cellular protein production. It plays a critical part in the ribosomal structure contributing to the ribosome's stability and function during translation. By binding to rRNA RPL30 ensures accurate alignment of ribosomal components supporting efficient and accurate protein synthesis. The protein is highly conserved and is ubiquitously expressed across various tissues and cell types due to its fundamental role.

Pathways

RPL30 is integral to the ribosome biogenesis and protein synthesis pathways. The protein interacts with other ribosomal proteins such as RPL10 and RPL5 to facilitate the assembly and function of the ribosome. These pathways are essential for cellular growth and proliferation influencing various biological processes including metabolism and cell cycle regulation. RPL30 works within these pathways to aid in the coordination of protein synthesis an activity critical for maintaining cellular homeostasis.

RPL30 is associated with diseases affecting protein synthesis such as ribosomopathies. Alterations or mutations in RPL30 can lead to defects in ribosome function contributing to conditions like Diamond-Blackfan anemia. This disorder is characterized by a failure in red blood cell production which can be linked to mutations in ribosomal proteins including RPS19. The relationship between RPL30 and these diseases highlights the importance of accurate ribosome function for maintaining normal cellular activities.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).
See full target information 60S ribosomal protein L30
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai
제품이 사용된 논문 모두 보기
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Product promise

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