JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317199

Anti-RPL32 antibody [HL2331]

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal RPL32 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Dog, Human, Zebrafish samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RPL32.

대체 명칭 보기

PP9932, RPL32, Large ribosomal subunit protein eL32, 60S ribosomal protein L32

7 이미지
Immunocytochemistry/ Immunofluorescence - Anti-RPL32 antibody [HL2331] (AB317199)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL32 antibody [HL2331] (AB317199)

ab317199 detects RPL32 protein at cytoplasm by immunofluorescent analysis. Sample : HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : RPL32 stained by ab317199 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody [HL2331] (AB317199)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody [HL2331] (AB317199)

ab317199 detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. RPL32 stained by ab317199 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody [HL2331] (AB317199)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody [HL2331] (AB317199)

ab317199 detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded rat brain. RPL32 stained by ab317199 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-RPL32 antibody [HL2331] (AB317199)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPL32 antibody [HL2331] (AB317199)

Immunofluorescence analysis of MDCK cells fixed in ice-cold MeOH for 5 min labelling RPL32 protein using ab317199 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution (Red).
DAPI was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody [HL2331] (AB317199)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody [HL2331] (AB317199)

ab317199 detects RPL32 protein by immunohistochemical analysis. Sample : Paraffin-embedded dog tissues. RPL32 stained by ab317199 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-RPL32 antibody [HL2331] (AB317199)
  • WB

Supplier Data

Western blot - Anti-RPL32 antibody [HL2331] (AB317199)

Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317199 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RPL32 antibody [HL2331] (ab317199) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RPL32 antibody [HL2331] (AB317199)
  • WB

Supplier Data

Western blot - Anti-RPL32 antibody [HL2331] (AB317199)

Whole cell extract (30 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317199 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RPL32 antibody [HL2331] (ab317199) at 1/1000 dilution

All lanes:

MDCK whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2331

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Dog, Zebrafish

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RPL32.

P62910

style
left-column

보완 제품

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB174318

Anti-RPL10A antibody [EPR12344]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (AB174318)

  • 4
  • 1
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB196652

Anti-RPS24 antibody [EPR16017(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (AB196652)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL32 also known as 60S ribosomal protein L32 is a component of the 60S large ribosomal subunit. Weighing approximately 13.5 kDa RPL32 plays a role in the assembly and function of ribosomes which are essential for protein synthesis. This protein is expressed ubiquitously in various tissues reflecting the constant need for ribosomal function across different cell types.
Biological function summary

Ribosomes are the machinery for translating mRNA into proteins in the cell. RPL32 forms part of the ribosomal complex engaging in the critical process of assembling amino acids into polypeptide chains. By contributing to ribosome stability and efficiency RPL32 ensures the fidelity and throughput of protein synthesis which is important for cellular growth and maintenance.

Pathways

Protein synthesis involves ribosome-mediated translation where RPL32 acts in coordination with other ribosomal proteins and rRNA. It integrates into the translation pathway working with key proteins like RPL10 and RPL5 to facilitate ribosomal activity. The interaction of these proteins influences the performance of ribosomes in synthesizing functional proteins needed for cellular processes including metabolism and stress response.

Disruptions in RPL32 function can lead to disease states connected to dysregulated protein synthesis. Research implicates RPL32 in certain malignancies particularly where ribosomal dysfunction contributes to unchecked cellular proliferation. Moreover RPL32 abnormalities show correlation with disorders like Diamond-Blackfan anemia which involves defective ribosomal function. The protein's relationship with other ribosomal constituents such as RPL24 suggests a complex network of interactions that can impact disease outcomes.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).
See full target information RPL32
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com