JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122255

Anti-RPL34 antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal RPL34 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L34 aa 1-100.

대체 명칭 보기

Large ribosomal subunit protein eL34, 60S ribosomal protein L34, RPL34

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-RPL34 antibody (AB122255)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL34 antibody (AB122255)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab122255 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL34 antibody (AB122255)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL34 antibody (AB122255)

ab122255, at 1/10, staining RPL34 in paraffin embedded Human pancreas tissue by Immunohistochemistry.

Western blot - Anti-RPL34 antibody (AB122255)
  • WB

Supplier Data

Western blot - Anti-RPL34 antibody (AB122255)

All lanes:

Western blot - Anti-RPL34 antibody (ab122255)

All lanes:

Marker (15, 25, 35, 55, 70, 100, 130, 250 kDa)

Predicted band size: 13 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human 60S ribosomal protein L34 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P49207

style
left-column

보완 제품

Primary Antibodies

AB86863

Anti-RPL5 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL5 antibody (AB86863)

  • 0
  • 0
Primary Antibodies

AB182556

Anti-RPL9 antibody [EP13752]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody [EP13752] (AB182556)

  • 0
  • 0
Primary Antibodies

AB155961

Anti-RPS2 antibody [EPR10834(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

  • 0
  • 0
Primary Antibodies

AB131459

Anti-S6K1 (phospho S411) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho S411) antibody (AB131459)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The RPL34 protein also known as ribosomal protein L34 is integral to the function of ribosomes where it plays a role in protein synthesis. It is a component of the 60S large ribosomal subunit and has a molecular mass of approximately 13 kDa. RPL34 is expressed in a wide range of tissues reflecting its essential role in the general process of translation. The conservation of RPL34 across species highlights its fundamental function in cellular machinery.
Biological function summary

RPL34 participates in the assembly and function of the ribosomal complex essential for translating mRNA into proteins. As part of the ribosome it interacts with rRNA and other ribosomal proteins to form a functional unit necessary for protein synthesis. The activity of RPL34 ensures accurate translation and effective cellular protein production reinforcing the ribosome's role as the cell's protein factory. Disruptions in its function can lead to impaired cellular processes due to defective protein production.

Pathways

RPL34 plays a vital role in the translation pathway where it ensures the correct assembly and function of the ribosomal complex. It is part of the ribosome biogenesis pathway which involves numerous other ribosomal proteins and factors. Its interaction with other ribosomal proteins such as RPL5 and RPL11 ensures efficient ribosomal assembly and function which is important for cellular homeostasis. The proper function of these pathways is important for maintaining cellular growth and division.

Mutations or dysregulation in RPL34 can be linked to ribosomopathies which include Diamond-Blackfan anemia a disorder characterized by anemia and congenital abnormalities. Research also suggests a connection between RPL34 and cancer where altered expression levels can contribute to tumorigenesis via the deregulation of ribosome biogenesis. The link with proteins like MDM2 involved in ribosome activity and cell cycle regulation further implicates RPL34 in pathways that could lead to disease when aberrantly expressed or mutated.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547).
See full target information 60S ribosomal protein L34
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Autophagy 20:1537-1558 PubMed38591519

2024

Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Macke,Taylor E Divita,Artem N Pachikov,Sundararajan Mahalingam,Ramesh Bellamkonda,Karuna Rasineni,Carol A Casey,Armen Petrosyan
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com