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AB241070

Anti-RPL35A antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal RPL35A antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RPL35A aa 50 to C-terminus.

대체 명칭 보기

GIG33, RPL35A, Large ribosomal subunit protein eL33, 60S ribosomal protein L35a, Cell growth-inhibiting gene 33 protein

1 이미지
Western blot - Anti-RPL35A antibody (AB241070)
  • WB

Supplier Data

Western blot - Anti-RPL35A antibody (AB241070)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPL35A antibody (ab241070) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 13 kDa

false

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RPL35A aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P18077

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보완 제품

Primary Antibodies

AB223767

Anti-DIS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

  • 0
  • 0
Primary Antibodies

AB15576

Anti-SEC61B antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB15576)

  • 4
  • 1
Primary Antibodies

AB5831

Anti-SMN/Gemin 1 antibody [2B1]

BOND RX™ Validated

Flow Cytometry - Anti-SMN/Gemin 1 antibody [2B1] (AB5831)

  • 5
  • 3
Assay Kits

AB253778

Human GAPDH Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human GAPDH Antibody Pair - BSA and Azide free (AB253778)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Antibody was affinity purified using an epitope specific to RPL35A immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL35A also known as 60S ribosomal protein L35a is a component of the ribosome particularly within the large 60S subunit. This protein has a mass of approximately 16.6 kDa and is expressed ubiquitously across various tissues consistent with its role in protein synthesis. The widespread expression indicates its importance in maintaining normal cell functions due to its involvement in ribosomal function.
Biological function summary

Researchers recognize RPL35A as a part of the ribosome complex which is essential for translation during protein synthesis. This complex participates directly in the assembly of polypeptide chains from mRNA transcripts. By engaging in this fundamental process RPL35A ensures that proteins are synthesized accurately and efficiently contributing to cellular homeostasis and growth.

Pathways

Researchers note that RPL35A plays a part in the mRNA translation pathway and the ribosome biogenesis pathway. Its function in assembling ribosomal subunits connects it to proteins like RPL11 and RPS19 which are also important components of the ribosome. These interconnected pathways facilitate the synthesis of proteins impacting cellular activities and overall organism development.

Scientists link RPL35A function to Diamond-Blackfan Anemia a rare genetic disorder that affects red blood cell production. In this condition mutations or deficiencies in RPL35A disrupt normal ribosome function leading to insufficient erythropoiesis. Additionally RPL35A interacts with other ribosomal proteins such as RPS19 another gene associated with Diamond-Blackfan Anemia emphasizing the complexity of ribosomal malfunctions in disease pathogenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547). Required for the proliferation and viability of hematopoietic cells (PubMed : 18535205).
See full target information RPL35A
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer medicine 14:e70985 PubMed40552444

2025

RPL35A Downregulation Suppresses Hepatocellular Carcinoma Cell Proliferation via NCAPG2 Inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,Yujie Lin,Yu Lai,Yanshan Li,Tao Chen,Xingxi Luo,Yibiao Ye

Cancer cell international 21:497 PubMed34535139

2021

RPL35A is a key promotor involved in the development and progression of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wu,Dachuan Sun,Yuqian Liao,Kai Shang,Canrong Lu

Cell death & disease 11:225 PubMed32269215

2020

Zinc finger protein 703 induces EMT and sorafenib resistance in hepatocellular carcinoma by transactivating CLDN4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Hongfa Xu,Feng Ma,Meixiao Zhan,Xiangyu Yang,Shengni Hua,Wei Li,Yong Li,Ligong Lu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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