AB241584

Anti-RPL36 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal RPL36 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RPL36 aa 50 to C-terminus.

대체 명칭 보기

Large ribosomal subunit protein eL36, 60S ribosomal protein L36, RPL36

2 이미지

Supplier Data

Immunoprecipitation - Anti-RPL36 antibody (AB241584)

RPL36 was immunoprecipitated from HeLa (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241584 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241584 at 1 μg/ml.

Lane 1 : ab241584 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RPL36 antibody (ab241584)

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-RPL36 antibody (AB241584)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RPL36 antibody (ab241584) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 12 kDa

true

Exposure time: 1s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPL36 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3U8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to RPL36 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPL36 also known as Ribosomal Protein L36 is a component of the 60S subunit of the eukaryotic ribosome. This protein has a mass of approximately 12.5 kDa. RPL36 helps in the assembly of ribosomes facilitating protein synthesis in the cell. It is expressed in various tissues including the liver and brain where cell growth and division require robust protein production.

Biological function summary

RPL36 plays an essential role in the synthesis of proteins by participating in ribosome biogenesis. As part of the ribosomal complex it contributes to the formation of the large ribosomal subunit which in collaboration with the small subunit synthesizes proteins from mRNA. This function places RPL36 at the heart of cellular physiology as protein synthesis drives many cellular processes.

Pathways

RPL36 is involved in the cellular process of translation. It is part of the ribosome-mediated mechanism that translates genetic information into proteins. RPL36 associates with other ribosomal proteins like RPL10 and RPL13A within the translational machinery. Additionally it is part of the mTOR signaling pathway which regulates cell growth proliferation and survival in response to nutrient availability and other signals.

RPL36 influences conditions such as Diamond-Blackfan anemia and various cancers. In Diamond-Blackfan anemia mutations affecting ribosomal proteins like RPL36 disrupt normal erythropoiesis leading to bone marrow failure. In cancer changes in ribosomal protein expression including RPL36 may contribute to abnormal protein synthesis and tumor growth. The interplay between RPL36 and other proteins such as RPL5 and RPL11 can impact pathways critical to disease development and progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547).

See full target information RPL36

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Disease markers 2022:5085183 PubMed36118675

2022

Curcumin Improves Keratinocyte Proliferation, Inflammation, and Oxidative Stress through Mediating the SPAG5/FOXM1 Axis in an Model of Actinic Dermatitis by Ultraviolet.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Chen,Yi Tang,Huiyan Deng,Bihua Liang,Huaping Li,Zhenjie Li,Huilan Zhu,Lezi Chen

iScience 24:102700 PubMed34235408

2021

Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Shadrach,Wesley M Stansberry,Allison M Milen,Rachel E Ives,Elizabeth A Fogarty,Anthony Antonellis,Brian A Pierchala

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL36 antibody

Immunoprecipitation - Anti-RPL36 antibody (AB241584)

Western blot - Anti-RPL36 antibody (AB241584)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise