AB102011

Anti-RPL9 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal RPL9 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus.

대체 명칭 보기

OK/SW-cl.103, RPL9P7, RPL9P8, RPL9P9, RPL9, Large ribosomal subunit protein uL6, 60S ribosomal protein L9

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody (AB102011)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody (AB102011)

Immunofluorescence analysis of methanol-fixed HeLa, using ab 102011 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Immunohistochemical analysis of paraffin embedded rt2 xenograft tissue with ab102011 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Immunohistochemical analysis of paraffin embedded mouse eye tissue stained for RPL9 protein at retinal pigment epithelium with ab102011 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Unknown

Western blot - Anti-RPL9 antibody (AB102011)

All lanes:

Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

HCT116 whole cell lysate at 30 µg

Predicted band size: 22 kDa

false

Unknown

Western blot - Anti-RPL9 antibody (AB102011)

All lanes:

Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution

All lanes:

mouse brain whole cell lysate at 50 µg

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-RPL9 antibody (AB102011)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-RPL9 antibody (ab102011) at 1/1000 dilution

All lanes:

Zebrafish muscle whole cell lysate at 30 µg

Predicted band size: 38 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Zebrafish

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RPL9 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P32969

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The RPL9 protein also known as Ribosomal Protein L9 plays a role in the structure and function of ribosomes. This protein is part of the large ribosomal subunit facilitating the assembly of proteins. With a molecular mass of approximately 21 kDa RPL9 resides in the cytoplasm where ribosomes are largely found reflecting its function. Broadly expressed across various tissues it is essential for ribosomal integrity and efficient protein synthesis.

Biological function summary

RPL9 is involved in ribosome biogenesis and translation processes. As a component of the ribosomal complex it aids in the production of new proteins by translating messenger RNA (mRNA) into polypeptides. This function is tightly regulated and essential for maintaining cellular protein homeostasis. Additionally RPL9 participates in ribosomal assembly ensuring the proper folding and function of ribosomes.

Pathways

RPL9 plays an integral part in the mRNA translation pathway. It associates with other ribosomal proteins such as RPL5 and RPL11 forming a functional ribosomal unit. RPL9 contributes to the translation of mRNA into proteins an indispensable step in various cellular growth and proliferation pathways. Its interactions with these pathways highlight its role in regulating protein synthesis necessary for cell function.

RPL9 has been linked to Diamond-Blackfan Anemia (DBA) and other ribosomopathies. These conditions arise from defects in ribosomal protein production leading to impaired hematopoiesis. RPL9 mutations could affect the assembly of ribosomes similar to other ribosomal proteins like RPS19. These diseases underline the importance of RPL9 in maintaining ribosome function and cellular health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 32669547).

See full target information RPL9

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 48:770-787 PubMed31799629

2019

Ribosomal protein gene RPL9 variants can differentially impair ribosome function and cellular metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Lezzerini,Marianna Penzo,Marie-Françoise O'Donohue,Carolina Marques Dos Santos Vieira,Manon Saby,Hyung L Elfrink,Illja J Diets,Anne-Marie Hesse,Yohann Couté,Marc Gastou,Alexandra Nin-Velez,Peter G J Nikkels,Alexandra N Olson,Evelien Zonneveld-Huijssoon,Marjolijn C J Jongmans,GuangJun Zhang,Michel van Weeghel,Riekelt H Houtkooper,Marcin W Wlodarski,Roland P Kuiper,Marc B Bierings,Jutte van der Werff Ten Bosch,Thierry Leblanc,Lorenzo Montanaro,Jonathan D Dinman,Lydie Da Costa,Pierre-Emmanuel Gleizes,Alyson W MacInnes

Journal of virology 87:1069-82 PubMed23135726

2012

Nucleolar trafficking of the mouse mammary tumor virus gag protein induced by interaction with ribosomal protein L9.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea R Beyer,Darrin V Bann,Breanna Rice,Ingrid S Pultz,Melissa Kane,Stephen P Goff,Tatyana V Golovkina,Leslie J Parent

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL9 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody (AB102011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody (AB102011)

Western blot - Anti-RPL9 antibody (AB102011)

Western blot - Anti-RPL9 antibody (AB102011)

Western blot - Anti-RPL9 antibody (AB102011)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise