JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB53744

Anti-RPP30 antibody [2931D5a]

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Mouse Monoclonal RPP30 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPP30.
2 이미지
Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)
  • WB

Unknown

Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)

All lanes:

Western blot - Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

anti Mouse IgG at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

true

Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)
  • WB

Unknown

Western blot - Anti-RPP30 antibody [2931D5a] (AB53744)

All lanes:

Western blot - Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution

All lanes:

NIH3T3 whole cell lysate at 25 µg

Secondary

All lanes:

anti Mouse IgG at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

true

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2931D5a

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RPP30. The exact immunogen used to generate this antibody is proprietary information.

P78346

style
left-column

보완 제품

Primary Antibodies

AB126338

Anti-RPP25 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (AB126338)

  • 0
  • 0
Primary Antibodies

AB75853

Anti-beta 2 Microglobulin antibody [EP2978Y]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] (AB75853)

  • 4
  • 9
Primary Antibodies

AB240640

Anti-CHD4 antibody [EPR22953-38] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (AB240640)

  • 0
  • 0
Primary Antibodies

AB219649

Anti-Vinculin antibody [EPR20407] - Loading Control

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - Loading Control (AB219649)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPP30 also known as Ribonuclease P protein subunit p30 is a protein involved in the enzymatic process that removes 5' leader sequences from precursor tRNA molecules. It has a mass of approximately 25 kDa. RPP30 is expressed in various tissues across the human body indicating its fundamental role in RNA processing.
Biological function summary

RPP30 plays a role as part of the Ribonuclease P complex which is important for the maturation of tRNA. The Ribonuclease P complex contains both RNA and protein components and RPP30 interacts with other subunits to form the complete ribonucleoprotein complex. This association is necessary for catalytic activity and stability of the complex.

Pathways

RPP30 is involved in RNA processing pathways important for cell viability. Specifically it is part of the tRNA processing pathway working alongside proteins like RPP14 and POP1 to assist in the maturation of tRNA. These interactions emphasize the integration of RPP30 in important cellular processes that affect protein synthesis.

Involvement of RPP30 in certain autoimmune conditions has been noted. Altered expression of RPP30 and its associated complex proteins can contribute to autoimmune diseases like systemic sclerosis. Additionally aberrations in RPP30 expression or function have been linked with alterations in RNA processing pathways that may impact cell proliferation and survival further showing connections to these disease states.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed : 30454648, PubMed : 9037013, PubMed : 9630247). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed : 28115465).
See full target information RPP30

대체 명칭 보기

RNASEP2, RPP30, Ribonuclease P protein subunit p30, RNaseP protein p30, RNase P subunit 2
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer management and research 12:8253-8261 PubMed32982421

2020

LncRNA GAS5 Regulates Osteosarcoma Cell Proliferation, Migration, and Invasion by Regulating RHOB via Sponging miR-663a.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Yao,Xianan Li,Yi Luo,Xuezheng Xu,Jianfan Liu,Jie Bu
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com