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AB17996

Anti-RPRD2 antibody

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Goat Polyclonal RPRD2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPRD2 aa 1000 to C-terminus.

대체 명칭 보기

KIAA0460, HSPC099, RPRD2, Regulation of nuclear pre-mRNA domain-containing protein 2

1 이미지
Western blot - Anti-RPRD2 antibody (AB17996)
  • WB

Unknown

Western blot - Anti-RPRD2 antibody (AB17996)

Detection of Human RPRD2 by Western Blot.

Samples : Whole cell lysate (15 and 50 μg) from HEK293 cells.

Antibody : Affinity purified goat anti-RPRD2 antibody used at 1 and 0.4 mg/ml.

Detection : Chemiluminescence with an exposure time of 1 minute.

Lanes 1 - 2:

Western blot - Anti-RPRD2 antibody (ab17996) at 1 µg/mL

Lanes 3 - 4:

Western blot - Anti-RPRD2 antibody (ab17996) at 0.4 µg/mL

Lanes 1 and 3:

Whole cell lysate from HEK239 cells at 15 µg

Lanes 2 and 4:

Whole cell lysate from HEK239 cells at 50 µg

Predicted band size: 156 kDa

Observed band size: 225 kDa

false

Exposure time: 1min

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RPRD2 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5VT52

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Antibody was affinity purified using an epitope specific to RPRD2 immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPRD2 also known as Regulation of Nuclear Pre-mRNA Domain-Containing Protein 2 is involved in transcription regulation. It has a molecular weight of approximately 70 kDa and is expressed in various tissues with higher levels found in the liver and kidney. Mechanically RPRD2 interacts with the carboxyl-terminal domain of RNA polymerase II acting as a transcriptional regulator by facilitating the dephosphorylation process. This interaction influences the transcription cycle highlighting its role in gene expression regulation.
Biological function summary

The regulatory activities of RPRD2 impact cellular processes through its part in multiple protein complexes particularly those focusing on transcription and RNA processing. RPRD2 collaborates with other proteins such as RPRD1A and RPRD1B to form a complex involved in mediating interactions with transcription factors and phosphatases. This complex functions in a coordinated manner to regulate the transcription machinery and contribute to RNA polymerase II activity modulation which in turn affects gene expression at a broader level.

Pathways

RPRD2 participates in several critical pathways including the RNA polymerase II transcription pathway and the mRNA maturation pathway. RPRD2's connection with the phosphatase complex places it in a network of interactions vital to maintaining transcriptional fidelity. Additionally RPRD2 is related to pathways involving proteins such as CDK9 which plays a role in controlling pause release at transcription start sites. These interactions position RPRD2 as an essential mediator within transcriptional networks.

RPRD2 has connections to cancer and neurodegenerative disorders. Its role in transcription regulation links it to tumor development with dysregulation potentially contributing to oncogenesis. Additionally the interaction with proteins like RNA polymerase II associates RPRD2 with neurodegenerative diseases where transcriptional dysregulation often plays a significant role. These connections highlight RPRD2 as a significant target for further research in understanding complex diseases.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

CNS neuroscience & therapeutics 31:e70279 PubMed39968701

2025

NONHSAT141192.2 Facilitates the Stemness and Radioresistance of Glioma Stem Cells via the Regulation of PIK3R3 and SOX2.

Applications

Unspecified application

Species

Unspecified reactive species

Sihan Wang,Haolang Ming,Zhen Wang,Xingye Zhai,Xinyue Zhang,Di Wu,Yin Bo,Hang Wang,Yuanbo Luo,Zhenfeng Han,Lingyu Hao,Yijia Xiang,Xu Han,Zengguang Wang,Yi Wang
제품이 사용된 논문 모두 보기

Product promise

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