AB246916

Anti-RPS14 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal RPS14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPS14 aa 1-150.

대체 명칭 보기

PRO2640, RPS14, Small ribosomal subunit protein uS11, 40S ribosomal protein S14

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human fallopian tube tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human colon tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human cerebral cortex tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RPS14 antibody (AB246916)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS14 antibody (AB246916)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling RPS14 using ab246916 at 4 μg/ml (green) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Paraffin-embedded human skin tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-RPS14 antibody (AB246916)

Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : Human cell line RT-4

All lanes:

Western blot - Anti-RPS14 antibody (ab246916) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 16 kDa

false

Unknown

Western blot - Anti-RPS14 antibody (AB246916)

All lanes:

Western blot - Anti-RPS14 antibody (ab246916) at 0.4 µg/mL

All lanes:

Mouse cerebral cortex tissue lysate

Predicted band size: 16 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RPS14 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P62263

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ribosomal Protein S14 (RPS14) also known as 40S ribosomal protein S14 plays an important mechanical role in ribosome assembly. This protein is a component of the small 40S subunit of the ribosome and has a molecular mass of approximately 17 kDa. RPS14 is expressed in various tissues reflecting its importance in general protein synthesis. Its expression in these tissues ensures that the ribosomes can function efficiently to translate mRNA into proteins a necessary process for cellular function and growth.

Biological function summary

RPS14 contributes to ribosome biogenesis and is essential for proper ribosome function. It is part of the ribosomal complex and interacts with ribosomal RNA (rRNA) to help assemble the small subunit of the ribosome. This ensures that the ribosome can accurately process genetic information into polypeptides. The protein's biological role reflects its contribution to maintaining cellular homeostasis and supporting cellular growth through effective protein production.

Pathways

RPS14 has roles in pathways that are important for protein synthesis and cell cycle progression. It plays a part in the ribosome biogenesis pathway which is related to the production and processing of rRNA and works with other ribosomal proteins like RPS19. Additionally it is involved in the p53 signaling pathway where it interacts with proteins that regulate the cell cycle and apoptosis emphasizing its importance in cellular response to stress or damage.

Alterations in RPS14 are linked to specific blood conditions such as myelodysplastic syndromes especially those with deletion of chromosome 5q (5q- syndrome). The protein's alteration affects ribosome function and subsequently protein synthesis. This connection to 5q- syndrome involves interactions with other proteins such as RPS19 which also plays a role in ribosomal activities. Understanding RPS14’s impact on diseases aids in exploring therapeutic targets for related disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS14

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cells international 2025:5091529 PubMed40476182

2025

miR-1275 Delivered via Mesenchymal Stem Cell-Derived Extracellular Vesicles Regulates ER-Phagy Through AXIN2 in Nucleus Pulposus Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwu Dong,Hailong Zhang,Wenwei Yang,Keliang Huang,Xin Zhang,Lianxiang Xing,Ying Zhang,Kewen Zhao

Cell proliferation 56:e13458 PubMed36977657

2023

Rps14 upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Changling Xu,Jieyu Qi,Xiaojie Hu,Liyan Zhang,Qiuhan Sun,Nianci Li,Xin Chen,Fangfang Guo,Peina Wu,Yi Shi,Renjie Chai

Frontiers in cell and developmental biology 9:618899 PubMed33644057

2021

Cisplatin-Resistant Gastric Cancer Cells Promote the Chemoresistance of Cisplatin-Sensitive Cells via the Exosomal RPS3-Mediated PI3K-Akt-Cofilin-1 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yao Sun,Bo Xu,Qiu-Xue Wu,Wen-Lian Chen,Si Cai,Hui Zhang,Qing-Feng Tang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Immunocytochemistry/ Immunofluorescence - Anti-RPS14 antibody (AB246916)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS14 antibody (AB246916)

Western blot - Anti-RPS14 antibody (AB246916)

Western blot - Anti-RPS14 antibody (AB246916)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise