AB155961

Anti-RPS2 antibody [EPR10834(B)]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal RPS2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

대체 명칭 보기

RPS4, RPS2, Small ribosomal subunit protein uS5, 40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling RPS2 with ab155961 at a 1/1000 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Immunofluorescence analysis of HeLa cells labeling RPS2 with ab155961 at a 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling RPS2 with ab155961 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

All lanes:

Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 31 kDa

false

Lab

Western blot - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Lane 6:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa

false

Exposure time: 15s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10834(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Use of an HRP/AP polymerized secondary antibody recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPS2 also known as Ribosomal Protein S2 is a component of the 40S subunit of the ribosome. This protein plays a mechanical role in ribosome assembly and protein synthesis. RPS2 has a molecular mass of approximately 29 kDa. Researchers have observed its expression in various tissues indicating its involvement in general cellular processes. It facilitates the initiation of translation by binding to ribosomal RNA and other ribosomal proteins to form a functional ribosome.

Biological function summary

Ribosomal Protein S2 contributes significantly to ribosome function and protein translation. As part of the ribosome RPS2 aids in the decoding of messenger RNA (mRNA) into proteins which occurs in all cellular environments. This protein works in conjunction with other ribosomal proteins and rRNA to ensure accuracy and efficiency in protein synthesis. It forms an integral part of the ribosomal machinery that also includes many other proteins and RNA molecules congregating to form the translation initiation complex.

Pathways

Several biological systems depend on the role that Ribosomal Protein S2 plays in protein synthesis. The mTOR signaling pathway is particularly important as RPS2 and other ribosomal proteins function under the regulation of mTOR to control protein translation rates. Additionally RPS2 connects with proteins like eIF2 to influence translation initiation. Together they regulate critical processes such as cell growth and proliferation linking ribosomal function with cellular metabolic status.

Impaired Ribosomal Protein S2 function can lead to conditions such as Diamond-Blackfan anemia which results from defects in ribosome biogenesis. Mutations or disruptions in related proteins like RPS19 are often studied alongside RPS2 for their roles in hereditary and acquired anemias. Furthermore aberrant RPS2 expression can contribute to tumorigenesis implicating it in cancer studies where proteins like p53 may interact or affect its function revealing the broader implications of ribosomal proteins in human health and diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (PubMed : 23636399). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (PubMed : 23636399). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed : 23636399). Plays a role in the assembly and function of the 40S ribosomal subunit (By similarity). Mutations in this protein affects the control of translational fidelity (By similarity). Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity).

See full target information RPS2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta pharmaceutica Sinica. B 13:598-617 PubMed36873185

2023

Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in -mutated acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Feng,Yefan Han,Anni Hu,Yi Qu,Yili Hu,Hao Wu,Xinzhi Wang,Li He

BMC biology 20:276 PubMed36482461

2022

An IGF1-expressing endometrial stromal cell population is associated with human decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Wei Shi,Zhen-Zhen Lai,Hui-Li Yang,Wen-Jie Zhou,Xiao-Ya Zhao,Feng Xie,Song-Ping Liu,Wei-Dong Chen,Tao Zhang,Jiang-Feng Ye,Xiang-Yu Zhou,Ming-Qing Li

Molecular biology of the cell 31:1879-1891 PubMed32520643

2020

Recognition of nuclear export signals by CRM1 carrying the oncogenic E571K mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan M Baumhardt,Janek S Walker,Yoonji Lee,Binita Shakya,Chad A Brautigam,Rosa Lapalombella,Nick Grishin,Yuh Min Chook

Aging cell 16:1381-1393 PubMed28994181

2017

Age-associated dysregulation of protein metabolism in the mammalian oocyte.

Applications

Species

Mouse

Francesca E Duncan,Susmita Jasti,Ariel Paulson,John M Kelsh,Barbara Fegley,Jennifer L Gerton

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS2 antibody [EPR10834(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Western blot - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

Western blot - Anti-RPS2 antibody [EPR10834(B)] (AB155961)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise