AB268130

Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1]

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(2 제품이 사용된 논문 )

Mouse Monoclonal YWHAE antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human YWHAE.

대체 명칭 보기

14-3-3 protein epsilon, 14-3-3E, YWHAE

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)

Formalin-fixed, paraffin-embedded human testis tissue stained for RPS23 using ab268130 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)

Protein Array

Unknown

Protein Array - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)

Protein Array containing more than 19,000 full-length human proteins using ab268130.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CPTC-YWHAE-1

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

WB, Protein Array, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human YWHAE.

P62258

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from Bioreactor concentrate

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ribosomal protein S23 (RPS23) is an important component of the 40S ribosomal subunit which plays a central role in protein synthesis. Also known simply as S23 this protein has a mass of approximately 15 kDa. RPS23 is ubiquitously expressed in different tissues reflecting its essential role in cellular function. It is highly conserved across species highlighting its importance in fundamental biological processes.

Biological function summary

RPS23 is critical in the assembly and structural stability of ribosomes. Within the ribosomal complex it contributes to the accurate decoding of messenger RNA (mRNA) during translation. RPS23 forms part of the small ribosomal subunit which recognizes and binds mRNA and transfer RNA (tRNA). Its position and structure facilitate correct alignment of the mRNA ensuring precise protein synthesis.

Pathways

RPS23 has essential roles in the protein synthesis pathway and is involved in the translation initiation complex. It interacts closely with other ribosomal proteins like RPS19 to enable the production of polypeptide chains from mRNA templates. Through this process RPS23 connects to cellular growth and differentiation pathways such as the mTOR signaling pathway which influences protein biosynthesis and cell survival.

Abnormalities in RPS23 can lead to disorders such as ribosomopathies and cancer. Mutations in RPS23 affect ribosome function and efficiency linking it to diseases that involve rapid cell proliferation. In cancer alterations in ribosomal biogenesis often correlate with aggressive tumor growth where RPS23 may interact with proteins such as p53 that regulate cell cycle and apoptosis. This highlights the significance of maintaining normal RPS23 functions for cellular homeostasis and disease prevention.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 21189250). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 35343654). Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed : 12917326). Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI (PubMed : 37555661). Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection (PubMed : 22607805). Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination (PubMed : 37599448).

See full target information YWHAE

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC nephrology 25:387 PubMed39478440

2024

The prognostic role of activation of the complement pathways in the progression of advanced IgA nephropathy to end-stage renal disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Shimin Jiang,Dingxin Di,Guming Zou,Hongmei Gao,Shunlai Shang,Wenge Li

Functional & integrative genomics 23:16 PubMed36562844

2022

Integrated analysis of miRNA-mRNA regulatory network and functional verification of miR-338-3p in coronary heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Qi,Wenqi Han,Nier Zhong,Qiling Gou,Chaofeng Sun

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)

Protein Array - Anti-14-3-3E / Tryptophan 5-Monooxygenase Antibody [CPTC-YWHAE-1] (AB268130)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise