AB241405

Anti-RPS28 antibody

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Rabbit Polyclonal RPS28 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPS28 aa 1 to C-terminus.

대체 명칭 보기

Small ribosomal subunit protein eS28, 40S ribosomal protein S28, RPS28

2 이미지

Supplier Data

Immunoprecipitation - Anti-RPS28 antibody (AB241405)

RPS28 was immunopreciptated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241405 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab241405 at 0.4 μg/mL.

Lane 1 : ab241405 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.
Prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-RPS28 antibody (ab241405)

Predicted band size: 8 kDa

false

Supplier Data

Western blot - Anti-RPS28 antibody (AB241405)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPS28 antibody (ab241405) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 8 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RPS28 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62857

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to RPS28 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPS28 also known as ribosomal protein S28 is a component of the 40S subunit of the ribosome. The protein has a molecular mass of about 7.6 kDa and is part of the S28E family of ribosomal proteins. RPS28 is highly expressed in various tissues reflecting its importance in cells that are actively synthesizing proteins. This protein plays a mechanical role in ribosome function by participating in the assembly of ribosomal structures and facilitating translation.

Biological function summary

Ribosomal proteins like RPS28 are essential for protein biosynthesis. RPS28 is a part of the ribosomal small subunit complex which helps decode messenger RNA (mRNA) into polypeptides. Proper functioning of the ribosome ensures the accurate translation of genetic material into proteins which is necessary for cell growth and maintenance. The protein cooperates with other ribosomal proteins to maintain the integrity and functionality of the ribosomal machinery.

Pathways

RPS28 is involved in the translation pathway which is critical for gene expression. It contributes to the initiation phase of translation working alongside the eukaryotic initiation factor 3 (eIF3) complex. The protein also plays a role in the maturation of ribosomal RNA (rRNA) within the ribosome biogenesis pathway. Through these pathways RPS28 interacts with other ribosomal proteins such as RPS15 and RPS23 which are essential for the assembly and function of the ribosome.

Mutations in RPS28 have been linked to Diamond-Blackfan anemia a rare genetic condition characterized by bone marrow failure and anemia. This disorder demonstrates the protein's importance in hematopoiesis and red blood cell production. Additionally researchers are examining connections between RPS28 and its interaction with the p53 pathway given p53's role in tumor suppression and the DNA damage response suggesting potential implications in certain cancers. Understanding these interactions may contribute to better insights into the molecular basis of these diseases and potential therapeutic approaches.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the small ribosomal subunit (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 25901680, PubMed : 25957688). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RPS28

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research : CR 41:17 PubMed34998399

2022

The RNA-binding protein GRSF1 promotes hepatocarcinogenesis via competitively binding to YY1 mRNA with miR-30e-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Han,Chen Huang,Xiaofei Wang,Dongdong Tong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS28 antibody

Immunoprecipitation - Anti-RPS28 antibody (AB241405)

Western blot - Anti-RPS28 antibody (AB241405)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise