AB226356

Anti-RPS3A antibody - N-terminal

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Rabbit Polyclonal RPS3A antibody. N-terminal. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPS3A aa 1-50.

대체 명칭 보기

FTE1, MFTL, RPS3A, Small ribosomal subunit protein eS1, 40S ribosomal protein S3a, v-fos transformation effector protein, Fte-1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody - N-terminal (AB226356)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody - N-terminal (AB226356)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for RPS3A using ab226356 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-RPS3A antibody - N-terminal (AB226356)

RPS3A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226356 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226356 at 0.4 μg/ml.

Lane 1 : ab226356 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-RPS3A antibody - N-terminal (ab226356)

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-RPS3A antibody - N-terminal (AB226356)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-RPS3A antibody - N-terminal (ab226356) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 30 kDa

true

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human RPS3A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P61247

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab226356 was affinity purified using an epitope specific to RPS3A immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RPS3A also known as Ribosomal Protein S3A is a component of the 40S subunit of the ribosome and has a molecular mass of approximately 29 kDa. The protein interacts directly with mRNA and transfer RNA during protein synthesis. RPS3A is expressed in various tissues including the liver spleen and muscle indicating its widespread presence where protein synthesis is highly active.

Biological function summary

Ribosomal Protein S3A plays a role in the assembly and stability of the small ribosomal subunit. It forms part of the ribosomal complex important for decoding mRNA into polypeptides. RPS3A is also involved in regulating cell growth and proliferation cell cycle progression and apoptosis linking it to cell survival and maintenance. As a ribosomal protein it associates with other ribosomal proteins and rRNA within the ribosome.

Pathways

Ribosomal Protein S3A engages in processes like the mRNA translation pathway and the ribosome biogenesis pathway. It interacts with proteins such as eukaryotic translation initiation factors ensuring the correct initiation and regulation of translation. RPS3A also associates with proteins involved in the mTOR signaling pathway which influences cell growth and metabolism by regulating protein synthesis.

Ribosomal Protein S3A has been implicated in cancer especially hepatocellular carcinoma suggesting a potential role in tumorigenesis. Abnormal expression levels of RPS3A may lead to dysregulated cell proliferation. Additionally RPS3A is associated with Diamond-Blackfan Anemia where changes in its expression could contribute to impaired ribosome function. In these contexts it may interact with proteins like RPL11 and p53 known for their roles in cellular growth regulation and genomic stability.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). May play a role during erythropoiesis through regulation of transcription factor DDIT3 (By similarity).

See full target information RPS3A

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC cancer 24:532 PubMed38671389

2024

Desmoglein 2 and desmocollin 2 depletions promote malignancy through distinct mechanisms in triple-negative and luminal breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Yuan Han,Na Che,Jing Mo,Dan-Fang Zhang,Xiao-Hui Liang,Xue-Yi Dong,Xiu-Lan Zhao,Bao-Cun Sun

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS3A antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3A antibody - N-terminal (AB226356)

Immunoprecipitation - Anti-RPS3A antibody - N-terminal (AB226356)

Western blot - Anti-RPS3A antibody - N-terminal (AB226356)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise