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AB250828

Anti-RRP4 antibody [EPR13606(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal RRP4 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

대체 명칭 보기

RRP4, EXOSC2, Exosome complex component RRP4, Exosome component 2, Ribosomal RNA-processing protein 4

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13606(B)] - BSA and Azide free (AB250828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13606(B)] - BSA and Azide free (AB250828)

This data was developed using ab185235, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RRP4 with ab185235 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-RRP4 antibody [EPR13606(B)] - BSA and Azide free (AB250828)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RRP4 antibody [EPR13606(B)] - BSA and Azide free (AB250828)

This data was developed using ab185235, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling RRP4 with ab185235 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-RRP4 antibody [EPR13606(B)] - BSA and Azide free (AB250828)
  • WB

Supplier Data

Western blot - Anti-RRP4 antibody [EPR13606(B)] - BSA and Azide free (AB250828)

This data was developed using ab185235, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (<a href='/ko/products/primary-antibodies/rrp4-antibody-epr13606b-c-terminal-ab185235'>ab185235</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Unconjugated

    Anti-RRP4 antibody [EPR13606(B)] - C-terminal

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13606(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB166630

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker (AB166630)

  • 5
  • 2
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Sample Prep & Detection Kits

AB64238

DAB Substrate Kit

Immunohistochemistry - DAB Substrate Kit (AB64238)

  • 5
  • 7
Assay Kits

AB219926

Glutathione Peroxidase Activity Assay Kit (Fluorometric)

Functional Studies - Glutathione Peroxidase Activity Assay Kit (Fluorometric) (AB219926)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

ab250828 is the carrier-free version of ab185235.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RRP4 also known as RRP4 homolog is a component of the exosome complex a multi-protein complex involved in RNA processing. RRP4 plays an important mechanical role in RNA degradation and it is essential for maintaining RNA stability by facilitating the 3' to 5' exonucleolytic trimming of RNA molecules. This protein has a molecular mass of approximately 26 kDa and is widely expressed in the nucleus and cytoplasm where it participates in various RNA processing tasks.
Biological function summary

RRP4 performs an important role in RNA metabolism and is a vital constituent of the exosome complex which is responsible for RNA processing degradation and surveillance. The exosome complex acts in RNA quality control targeting malformed RNAs for degradation. RRP4 contributes to rRNA processing and maturation by forming stable interactions within the nucleolus. This activity ensures the accuracy and functionality of ribosomal RNA supporting the cell's protein synthesis machinery.

Pathways

RRP4 plays a critical function in the RNA processing pathways and is involved in rRNA and mRNA turnover. RRP4 interconnects with pathways involving exosome components like RRP6 and DIS3 which also contribute to RNA decay. Processing of rRNA by mechanisms involving RRP4 ensures the quality of ribosome assembly which is essential for translation and cellular homeostasis.

Improper function of the exosome complex including RRP4 associates with neurological conditions such as pontocerebellar hypoplasia and certain forms of cancer. RRP4 alongside proteins like RRP6 and DIS3 can accumulate mutations affecting exosome functionality. This can lead to disrupted RNA processing and accumulation of faulty RNA products contributing to disease pathogenesis. Understanding these interactions is vital for developing therapeutic approaches targeting such conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7.
See full target information EXOSC2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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