AB254600

Anti-RSU1 antibody

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Rabbit Polyclonal RSU1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RSU1 aa 100-300.

대체 명칭 보기

RSP1, RSU1, Ras suppressor protein 1, RSP-1, Rsu-1

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSU1 antibody (AB254600)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSU1 antibody (AB254600)

Paraffin-embedded human liver tissue stained for RSU1 using ab254600 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RSU1 antibody (AB254600)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RSU1 antibody (AB254600)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling RSU1 using ab254600 at 4µg/ml (green) in ICC/IF.

Supplier Data

Western blot - Anti-RSU1 antibody (AB254600)

All lanes:

Western blot - Anti-RSU1 antibody (ab254600) at 0.4 µg/mL

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-RSU1 antibody (AB254600)

All lanes:

Western blot - Anti-RSU1 antibody (ab254600) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 32 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RSU1 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q15404

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The RSU1 protein also referred to as Ras suppressor protein 1 is an influential regulator of cell adhesion and migration. It has a molecular mass of approximately 33 kDa. RSU1 mainly expresses in the brain and other tissues where it participates in various signaling pathways. The protein interfaces with integrin-linked kinase (ILK) and modulates actin cytoskeleton organization impacting cellular motility and interactions with the extracellular environment.

Biological function summary

The RSU1 protein integrates into a complex alongside ILK and other partners to affect cytoskeletal dynamics. It impacts cellular processes such as spreading adhesion and migration by modulating focal adhesion structures. RSU1 balances signaling cascades that influence cellular tension and stability contributing to cellular responses to external signals. This involvement makes RSU1 a notable player in maintaining cellular architecture and function.

Pathways

Involvement of RSU1 in the integrin and Rac1 signaling pathways highlights its functional roles. Through its interaction with ILK and p21-activated kinase (PAK) RSU1 regulates important steps in these pathways. The integration of RSU1 into these pathways underlines its contribution to cell shape modulation and movement emphasizing its role in normal cellular processes and homeostasis.

RSU1 research connects it with cancer progression and neurodegenerative diseases. Its dysregulation associates with altered cell migration and adhesion which are critical in cancer metastasis. RSU1 also engages with proteins like ILK which are implicated in tumorigenic pathways. In neurodegenerative contexts altered RSU1 expression patterns might affect neuronal structure and function linking it to conditions like Alzheimer's disease where cellular adhesion and cytoskeletal integrity are compromised.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Potentially plays a role in the Ras signal transduction pathway. Capable of suppressing v-Ras transformation in vitro.

See full target information RSU1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Life science alliance 6: PubMed37604583

2023

Tumor necrosis factor mediates USE1-independent FAT10ylation under inflammatory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Leonie Schnell,Alina Zubrod,Nicola Catone,Johanna Bialas,Annette Aichem

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RSU1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSU1 antibody (AB254600)

Immunocytochemistry/ Immunofluorescence - Anti-RSU1 antibody (AB254600)

Western blot - Anti-RSU1 antibody (AB254600)

Western blot - Anti-RSU1 antibody (AB254600)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise