AB242904

Anti-S100 antibody [EPR20041-32] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal S10A1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

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Sandwich ELISA - Anti-S100 antibody [EPR20041-32] - BSA and Azide free (Detector) (AB242904)

sELISA

Supplier Data

Sandwich ELISA - Anti-S100 antibody [EPR20041-32] - BSA and Azide free (Detector) (AB242904)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab219635).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20041-32

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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제품 세부 정보

ab242904 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human S100A1. The recommended pair for sandwich ELISA is:
Capture: ab242659, Human S100A1 Capture Antibody (unconjugated)
Detector: ab242904, Human S100A1 Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The S100 protein family consists of low-molecular-weight proteins with individual members like S100B and S100A4 each having distinct roles. S100 proteins range typically between 9 to 13 kDa in mass. They are expressed broadly but especially in the central nervous system where glial cells are an important source and in several epithelial tissues including melanocytes. These proteins are noted for their calcium-binding properties facilitated by EF-hand motifs influencing both intracellular and extracellular processes.

Biological function summary

S100 proteins drive diverse cellular functions such as regulation of cell cycle differentiation and motility. They often operate as dimers and participate in the formation of protein complexes impacting cellular signaling networks. By binding calcium S100 proteins undergo conformational changes that allow interaction with target proteins modulating processes like cytoskeleton dynamics and neuronal survival.

Pathways

S100 proteins are significant in the regulation of the MAPK/ERK and NF-kB pathways. These pathways are critical for cell proliferation apoptosis and response to environmental stress. S100B in particular interacts with the receptor for advanced glycation end-products (RAGE) influencing downstream signaling cascades and amplifying the cellular response to inflammatory stimuli.

S100 proteins are implicated in the progression of conditions such as melanoma and Alzheimer's disease. In melanoma S100B levels serve as a biomarker reflecting disease progression potentially interacting with p53 to diminish its tumor suppressor function. In Alzheimer's disease S100B overexpression correlates with neuroinflammation and neuronal damage highlighting its association with disease through pathways involving amyloid-beta and tau proteins.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).

See full target information S100A1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 antibody [EPR20041-32] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-S100 antibody [EPR20041-32] - BSA and Azide free (Detector) (AB242904)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise