AB240572

Anti-S100A16 antibody

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Polyclonal S100A16 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human S100A16 aa 50 to C-terminus.

대체 명칭 보기

S100F, AAG13, S100A16, Protein S100-A16, Aging-associated gene 13 protein, Protein S100-F, S100 calcium-binding protein A16

2 이미지

Supplier Data

Immunoprecipitation - Anti-S100A16 antibody (AB240572)

S100A16 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer using ab240572 at 20 μl/reaction. Western blot of the immunoprecipitates was performed using ab240572 at 1/100 dilution.

Lane 1 : ab240572 IP in HeLa whole cell lysate
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-S100A16 antibody (ab240572)

Predicted band size: 11 kDa

false

Supplier Data

Western blot - Anti-S100A16 antibody (AB240572)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-S100A16 antibody (ab240572) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 11 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human S100A16 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96FQ6

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use at 20 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

S100A16 also known as S100 calcium-binding protein A16 belongs to the S100 protein family. With a molecular mass of approximately 10.7 kDa S100A16 plays a role in the regulation of calcium homeostasis and cell differentiation. It is expressed in various tissues including the brain heart and adipose tissue. Like other S100 proteins S100A16 possesses two EF-hand motifs which facilitate its ability to bind to calcium ions influencing its structural conformation and function.

Biological function summary

S100A16 is involved in cellular processes such as proliferation differentiation and migration. It participates in the modulation of cytoskeletal dynamics and cell cycle regulation. S100A16 often integrates into multiprotein complexes where it interacts with target proteins to exert its biological effects. Studies show that S100A16 may also contribute to epithelial-mesenchymal transition (EMT) an important event in development and cancer progression.

Pathways

S100A16 functions in signaling pathways linked to calcium-dependent processes. In the MAPK signaling pathway it interacts with proteins like ERK1/2 to regulate cell proliferation and survival. Furthermore S100A16 is also associated with the TGF-beta pathway where its role in EMT becomes significant and involves interactions with TGF-beta-related factors. These pathways underline the importance of S100A16 in cellular homeostasis and developmental processes.

S100A16 is implicated in cancer and metabolic disorders. Its overexpression has been observed in several types of cancer including breast and colorectal cancer where it might contribute to tumor progression and metastasis. S100A16 also interacts with proteins like MMPs which are involved in tissue remodeling and invasion. Additionally S100A16 plays a part in metabolic diseases potentially through its role in adipocyte differentiation and lipid metabolism highlighting its multifaceted contribution to human health and disease.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Calcium-binding protein. Binds one calcium ion per monomer (PubMed : 17030513). Can promote differentiation of adipocytes (in vitro) (By similarity). Overexpression in preadipocytes increases their proliferation, enhances adipogenesis and reduces insulin-stimulated glucose uptake (By similarity).

See full target information S100A16

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine reports 31: PubMed39450567

2024

S100A16 stabilizes the ITGA3‑mediated ECM‑receptor interaction pathway to drive the malignant properties of lung adenocarcinoma cells via binding MOV10.

Applications

Unspecified application

Species

Unspecified reactive species

Lianren Yang,Ajuan Shen,Rujun Wang,Zhihui Zheng

Cell reports 33:108264 PubMed33053349

2020

Activation of the CARD8 Inflammasome Requires a Disordered Region.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley J Chui,Andrew R Griswold,Cornelius Y Taabazuing,Elizabeth L Orth,Kuo Gai,Sahana D Rao,Daniel P Ball,Jeffrey C Hsiao,Daniel A Bachovchin

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100A16 antibody

Immunoprecipitation - Anti-S100A16 antibody (AB240572)

Western blot - Anti-S100A16 antibody (AB240572)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise