JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244279

Anti-S2P antibody

4

(5 리뷰들)

|

(0 출판물)

Rabbit Polyclonal S2P antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MBTPS2 aa 250-400.

대체 명칭 보기

S2P, MBTPS2, Membrane-bound transcription factor site-2 protease, Endopeptidase S2P, Sterol regulatory element-binding proteins intramembrane protease, SREBPs intramembrane protease

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S2P antibody (AB244279)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S2P antibody (AB244279)

Formalin-fixed, paraffin-embedded human rectum tissue stained for SP2 using ab244279 at 1/20 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MBTPS2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

O43462

style
left-column

보완 제품

Primary Antibodies

AB30682

Anti-SREBP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

  • 4
  • 12
Primary Antibodies

AB190103

Anti-SCAP antibody

Flow Cytometry (Intracellular) - Anti-SCAP antibody (AB190103)

  • 0
  • 0
Primary Antibodies

AB224618

Anti-Site-1 Protease antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Site-1 Protease antibody - C-terminal (AB224618)

  • 4
  • 3
Primary Antibodies

AB313881

Anti-SREBP1 antibody [EPR27079-194]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-SREBP1 antibody [EPR27079-194] (AB313881)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

S2P also known as Site-2 protease or IMP1 is an intramembrane zinc metalloprotease with a mass around 52 kDa. It carries out the proteolytic cleavage of membrane-bound transcription factors. S2P resides in cellular locations like the endoplasmic reticulum membrane and is highly expressed in tissues such as the liver and muscle. This protease acts after the initial cleavage by Site-1 protease (S1P) playing an important role in regulated intramembrane proteolysis.
Biological function summary

S2P cleaves specific substrates involved in lipid metabolism and stress response. This protease is an integral component of the sterol-regulatory element-binding proteins (SREBPs) complex. The cleavage by S2P releases the active form of SREBPs which translocates into the nucleus to regulate genes responsible for cholesterol and fatty acid synthesis. S2P ensures that these transcription factors maintain lipid homeostasis and adapt to cellular environment changes.

Pathways

S2P significantly influences cholesterol biosynthesis and unfolded protein response pathways. In the cholesterol biosynthesis pathway it regulates the activation of SREBPs which in turn controls cholesterol and lipid biosynthesis genes. Additionally in the unfolded protein response pathway it interacts with proteins such as ATF6 to help manage endoplasmic reticulum stress. These interactions highlight S2P's pivotal role in maintaining cellular balance under varying conditions.

S2P dysfunction can lead to conditions like hypercholesterolemia and metabolic syndrome. Impaired S2P activity disrupts normal cholesterol regulation potentially causing elevated cholesterol levels and related cardiovascular diseases. S2P also connects to ATF6 in response to stress and any disturbances might influence metabolic pathways contributing to metabolic syndrome. Understanding the role of S2P in these diseases offers insights into potential therapeutic approaches for managing lipid-related disorders.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Zinc metalloprotease that mediates intramembrane proteolysis of proteins such as ATF6, ATF6B, SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed : 10805775, PubMed : 11163209). Catalyzes the second step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 : cleaves SREBPs within the first transmembrane segment, thereby releasing the N-terminal segment with a portion of the transmembrane segment attached (PubMed : 10805775, PubMed : 27380894, PubMed : 9659902). Mature N-terminal SREBP fragments shuttle to the nucleus and activate gene transcription (PubMed : 10805775, PubMed : 27380894, PubMed : 9659902). Also mediates the second step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B) (PubMed : 11163209). Involved in intramembrane proteolysis during bone formation (PubMed : 27380894). In astrocytes and osteoblasts, upon DNA damage and ER stress, mediates the second step of the regulated intramembrane proteolytic activation of the transcription factor CREB3L1, leading to the inhibition of cell-cycle progression (PubMed : 16417584).
See full target information MBTPS2
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com