JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229609

Anti-SA2 antibody - C-terminal

Be the first to review this product! 리뷰 제출

|

(2 제품이 사용된 논문 )

Rabbit Polyclonal SA2 antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human STAG2.

대체 명칭 보기

SA2, STAG2, Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody - C-terminal (AB229609)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody - C-terminal (AB229609)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SA2 (green) using ab229609 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue). F-actin stained with Phalloidin (red).

Western blot - Anti-SA2 antibody - C-terminal (AB229609)
  • WB

Supplier Data

Western blot - Anti-SA2 antibody - C-terminal (AB229609)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SA2 antibody - C-terminal (ab229609) at 1/6000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

SA2 shRNA-transfected HEK-293T whole cell extract at 30 µg

Predicted band size: 141 kDa

true

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Synthetic Peptide within Human STAG2. The exact immunogen used to generate this antibody is proprietary information.

Q8N3U4

style
left-column

대체품

Primary Antibodies

AB171970

Anti-SA2 antibody [EPR10993] - N-terminal

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-SA2 antibody [EPR10993] - N-terminal (AB171970)

  • 0
  • 0
Primary Antibodies

AB201451

Anti-SA2 antibody [EPR17865] - C-terminal

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (AB201451)

  • 5
  • 2
Primary Antibodies

AB155081

Anti-SA2 antibody [EPR10994(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (AB155081)

  • 0
  • 0

보완 제품

Primary Antibodies

AB217678

Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

20ul selling size|BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody [EPR22506-15] - ChIP Grade (AB217678)

  • 4
  • 2
Primary Antibodies

AB4457

Anti-SA1 antibody

Immunocytochemistry - Anti-SA1 antibody (AB4457)

  • 0
  • 0
Primary Antibodies

AB128873

Anti-CTCF antibody [EPR7314(B)] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (AB128873)

  • 5
  • 1
Primary Antibodies

AB9263

Anti-SMC3 antibody

Immunoprecipitation - Anti-SMC3 antibody (AB9263)

  • 5
  • 7
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.
Biological function summary

SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.

Pathways

SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.

SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
See full target information STAG2
style
left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

World journal of gastrointestinal oncology 17:110527 PubMed40977645

2025

Transcription factor 3 enhances hepatocellular carcinoma metastasis by upregulating matrix metalloproteinase-11.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Peng Tian,Tian-Hao Wu,Guang-Jun Zhang,Sheng-Jie Li

Signal transduction and targeted therapy 7:192 PubMed35729157

2022

Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Tao Li,Hai Yang,Ming-Zhu Lei,Wei-Ping Zhu,Ying Su,Kai-Yue Li,Wen-Ying Zhu,Jian Wang,Lei Zhang,Jia Qu,Lei Lv,Hao-Jie Lu,Zheng-Jun Chen,Lu Wang,Miao Yin,Qun-Ying Lei
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com