AB247790

Anti-SAM68 antibody [EPR3232] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal SAM68 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

SAM68, KHDRBS1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, p21 Ras GTPase-activating protein-associated p62, p68, Sam68

4 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

This data was developed using ab109197, the same antibody clone in a different buffer formulation.ab109197, at 1/250, staining SAM68 in HeLa cells by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

This data was developed using ab109197, the same antibody clone in a different buffer formulation.

ab109197, at 1/250, staining SAM68 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

This data was developed using ab109197, the same antibody clone in a different buffer formulation.

Overlay histogram showing HeLa cells stained with ab109197 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109197, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Unknown

Western blot - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

This data was developed using ab109197, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SAM68 antibody [EPR3232] (<a href='/ko/products/primary-antibodies/sam68-antibody-epr3232-ab109197'>ab109197</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

A673 cell lysate at 10 µg

Predicted band size: 48 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3232

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab247790 is the carrier-free version of ab109197.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein SAM68 also known as Src-associated in mitosis 68 kDa is a member of the STAR (Signal Transduction and Activation of RNA) family and has a mass of approximately 68 kilodaltons. SAM68 is expressed in a broad range of tissues but shows higher expression in the brain and testis. It functions mechanically as a nuclear RNA-binding protein involved in RNA metabolism alternative splicing and is linked to signal transduction pathways. The ability of SAM68 to bind RNA is important for its role in RNA processing.

Biological function summary

SAM68 impacts multiple cellular processes by regulating alternative splicing of pre-mRNA and is known to be part of a larger ribonucleoprotein complex. This functionality enables SAM68 to influence gene expression and impact cell proliferation and differentiation. By interacting with several signaling molecules it modulates processes that are vital for the proper functioning of various cell types including neurons and germ cells.

Pathways

SAM68 interacts with several signaling pathways notably the PI3K/AKT and MAPK/ERK pathways where it influences cellular survival and growth. Within these pathways SAM68 interacts with proteins such as the tyrosine kinase Src which facilitates signal transduction events leading to changes in cell behavior. The protein also associates with hnRNPA1 affecting mRNA processing and influencing downstream signaling cascades.

SAM68 has connections to cancer and neurodegenerative diseases. Abnormal expression or mutations in SAM68 are linked to oncogenesis particularly in prostate and breast cancers where it interacts with signaling proteins like ERK. In neurodegenerative disorders SAM68 is implicated in altering neuronal RNA processing potentially contributing to diseases such as Alzheimer's with connections to proteins like tau. Understanding SAM68's role in these diseases highlights its potential as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed : 22253824). RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner (PubMed : 26080397). In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1 (PubMed : 17371836, PubMed : 20186123). Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (By similarity).. Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.

See full target information KHDRBS1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAM68 antibody [EPR3232] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

Flow Cytometry (Intracellular) - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

Western blot - Anti-SAM68 antibody [EPR3232] - BSA and Azide free (AB247790)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise