AB121831

Anti-SAMD12 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal SAMD12 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Sterile alpha motif domain-containing protein 12 aa 1-150.

대체 명칭 보기

Sterile alpha motif domain-containing protein 12, SAM domain-containing protein 12, SAMD12

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Immunohistochemical analysis of human pancreas tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human islets of Langerhans.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Immunohistochemical analysis of human fallopian tube tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-SAMD12 antibody (AB121831)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAMD12 antibody (AB121831)

Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121831 1-4 µg/ml. Cells treated with PFA/Triton X-100.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Immunohistochemical analysis of human cerebral cortex tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human neuronal cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Immunohistochemical analysis of human colon tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human glandular cells.

Unknown

Western blot - Anti-SAMD12 antibody (AB121831)

All lanes:

Western blot - Anti-SAMD12 antibody (ab121831) at 1/250 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

Over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Predicted band size: 23 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Sterile alpha motif domain-containing protein 12 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8N8I0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SAMD12 short for Sterile Alpha Motif Domain Containing 12 has a mechanical role in cellular processes and protein interactions. It is a protein with a mass of approximately 105 kDa. SAMD12 expression occurs broadly across human tissues with higher presence noted in the brain and testis. This protein belongs to the family of proteins containing sterile alpha motifs which are known for their involvement in protein-protein interactions.

Biological function summary

SAMD12 functions as a mediator in cellular signaling and communication. It participates in processes that regulate gene expression and cellular structure contributing to neuronal development and maintenance. Although SAMD12 is not part of a large complex it interacts with diverse proteins that aid in stabilizing cellular architecture and signaling networks.

Pathways

SAMD12 has significant interactions within the cellular signaling and development domains. It plays roles in the Wnt signaling pathway and neurodevelopmental pathways. SAMD12 interacts with proteins such as beta-catenin in the Wnt pathway influencing cellular proliferation and differentiation. Through these pathways SAMD12 connects to various proteins that coordinate cellular growth and structural integrity.

SAMD12 has associations with neurodegenerative disorders and certain cancers. In neurological contexts abnormal SAMD12 expression links to conditions like hereditary spastic paraplegia where it may interact with proteins like SPAST affecting neuronal function. SAMD12's involvement in cancers relates to its role in cellular signaling pathways that regulate cell growth and division suggesting its influence in tumorigenesis processes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Sterile alpha motif domain-containing protein 12

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 21:e1012467 PubMed40198713

2025

A targeted CRISPR screen identifies ETS1 as a regulator of HIV-1 latency.

Applications

Unspecified application

Species

Unspecified reactive species

Manickam Ashokkumar,Terry L Hafer,Abby Felton,Nancie M Archin,David M Margolis,Michael Emerman,Edward P Browne

Cancer discovery 13:332-347 PubMed36259929

2022

A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Fengbiao Zhou,Nesrine Aroua,Yi Liu,Christian Rohde,Jingdong Cheng,Anna-Katharina Wirth,Daria Fijalkowska,Stefanie Göllner,Michelle Lotze,Haiyang Yun,Xiaobing Yu,Caroline Pabst,Tim Sauer,Thomas Oellerich,Hubert Serve,Christoph Röllig,Martin Bornhäuser,Christian Thiede,Claudia Baldus,Michaela Frye,Simon Raffel,Jeroen Krijgsveld,Irmela Jeremias,Roland Beckmann,Andreas Trumpp,Carsten Müller-Tidow

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAMD12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Immunocytochemistry/ Immunofluorescence - Anti-SAMD12 antibody (AB121831)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

Western blot - Anti-SAMD12 antibody (AB121831)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise