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AB308371

Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443]

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Rabbit Recombinant Monoclonal VEMP antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2 samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within SARS-CoV-2 E.
1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 Envelope antibody [HL1443] (AB308371)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 Envelope using ab308371 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1443

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within SARS-CoV-2 E. The exact immunogen used to generate this antibody is proprietary information.

P0DTC4

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보완 제품

Sample Prep & Detection Kits

AB270709

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)

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Sample Prep & Detection Kits

AB154873

Gold Conjugation Kit (40nm, 20 OD)

Conjugation - Gold Conjugation Kit (40nm, 20 OD) (AB154873)

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  • 0
Primary Antibodies

AB271180

Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (AB271180)

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  • 4
Primary Antibodies

AB283946

Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174]

20ul selling size|RabMAb|Recombinant

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (AB283946)

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  • 1
Secondary Antibodies

AB150078

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (AB150078)

  • 5
  • 2
Primary Antibodies

AB280953

Anti-SARS-CoV2 orf3a antibody [EPR24848-9]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV2 orf3a antibody [EPR24848-9] (AB280953)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV envelope protein also known as E protein is a small membrane protein with a mass of approximately 9.6 kDa. It is a structural component of the SARS coronavirus and integrates into the viral envelope. This protein expresses primarily in the membranes of infected host cells contributing to virion assembly budding and release. The E protein acts as an ion channel and facilitates virus-to-host cell interaction which is critical during the coronavirus life cycle.
Biological function summary

The E protein functions within virus replication and pathogenesis. It forms part of the viral assembly complex interacting with the membrane (M) protein and nucleocapsid (N) protein during virion maturation. The E protein impacts the host cell’s cellular pathways by altering membrane permeability and modulating host cell stress responses. Additionally its ion channel activity contributes to virus-host cell interactions and is believed to play a role in immune response evasion.

Pathways

The E protein participates in the coronavirus replication and assembly process. It is involved in the virion assembly pathway and interacts with other viral proteins like the M and N proteins to facilitate this process. The E protein's role in the modification of the host cell’s Golgi apparatus assists in viral budding and release an essential part of the coronavirus replication cycle that is important for efficient viral propagation.

The E protein has significance in the pathogenesis of severe acute respiratory syndrome (SARS) and its role in coronavirus infections. Its interaction with other viral proteins like the structural M protein contributes to the inflammatory responses seen in SARS-CoV infections. Additionally due to its ion channel activity the E protein could influence respiratory complications by affecting cellular stress and apoptosis pathways associated with SARS-CoV related diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed : 33229438). May act by slowing down the cell secretory pathway (PubMed : 33229438). May interfere with tight-junction stability by interacting with host MPP5. This would result in disruption of epithelial barriers, thereby amplifying inflammatory processes (PubMed : 32891874).
See full target information E

대체 명칭 보기

4, E, Envelope small membrane protein, sM protein
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406211 PubMed39301916

2024

Lung-Targeted Lipid Nanoparticle-Delivered siUSP33 Attenuates SARS-CoV-2 Replication and Virulence by Promoting Envelope Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzheng Zhou,Yujie Liao,Lujie Fan,Xiafei Wei,Qiang Huang,Chuwei Yang,Wei Feng,Yezi Wu,Xiang Gao,Xiaotong Shen,Jian Zhou,Zanxian Xia,Zheng Zhang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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