![Indirect ELISA - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Human IgG1 (Chimeric) - BSA and Azide free (AB323002)](https://content.abcam.com/products/images/sars-cov-2-s-antibody-epr24852-116-human-igg1-chimeric-bsa-and-azide-free-ab323002--indirect-elisa-img440917.jpg/_jcr_content/renditions/webp-768.webp)
- I-ELISA
Lab
Indirect ELISA - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Human IgG1 (Chimeric) - BSA and Azide free (AB323002)
This data was developed using ab323000, the same antibody clone in a different buffer formulation.
Indirect ELISA showing primary antibody ab323000 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab323000 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1 : 5000.
![Western blot - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Human IgG1 (Chimeric) - BSA and Azide free (AB323002)](https://content.abcam.com/products/images/sars-cov-2-s-antibody-epr24852-116-human-igg1-chimeric-bsa-and-azide-free-ab323002--western-blot-img440918.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Lab
Western blot - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Human IgG1 (Chimeric) - BSA and Azide free (AB323002)
This data was developed using ab323000, the same antibody clone in a different buffer formulation.
Lane 1 : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (ab273068) at 0.2 ug
Lane 2 : Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 ug
Performed under reducing conditions.
Observed band size : 135 kDa.
False colour image of Western blot : Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - Human IgG1 (Chimeric), ab323000 staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, ab323000 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.
Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T).
Exposure time : 30 Sec.
All lanes:
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) (<a href='/ko/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-antibody-epr24852-116-human-igg1-chimeric-ab323000'>ab323000</a>) at 1/500 dilution
Lane 1:
Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (<a href='/ko/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a>) at 0.2 µg
Lane 2:
Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 µg
Secondary
Lanes 1 - 2:
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution
Lanes 1 - 2:
Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 135 kDa
false
Exposure time: 30s
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric)
Reactivity 정보
제품 세부 정보
This human monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab283942). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
ab323002 is the carrier free version of ab323000.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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