AB69995

Anti-SATB2 antibody

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(3 리뷰들)

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(7 제품이 사용된 논문 )

Rabbit Polyclonal SATB2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SATB2.

1 이미지

Western blot - Anti-SATB2 antibody (AB69995)

WB

Unknown

Western blot - Anti-SATB2 antibody (AB69995)

Lane 1:

Western blot - Anti-SATB2 antibody (ab69995) at 2 µg/mL

Lane 2:

Western blot - Anti-SATB2 antibody (ab69995) at 4 µg/mL

All lanes:

Mouse brain tissue lysate at 15 µg

Predicted band size: 83 kDa

Observed band size: 90 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

Immunogen

Synthetic Peptide within Human SATB2. The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab69995 does not cross-react with SATB1.

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대체품

Primary Antibodies

AB92446

Anti-SATB2 antibody [EPNCIR130A]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130A] (AB92446)

5
10

Primary Antibodies

AB225052

PE Anti-SATB2 antibody [EPNCIR130A]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-SATB2 antibody [EPNCIR130A] (AB225052)

0
0

Primary Antibodies

AB207040

Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (AB207040)

0
0

Primary Antibodies

AB212177

Anti-SATB2 antibody [EPNCIR130A] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130A] - BSA and Azide free (AB212177)

0
0

Primary Antibodies

AB196536

Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A] (AB196536)

0
0

Primary Antibodies

AB133328

Anti-SATB2 antibody [EPNCIR130B]

RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] (AB133328)

0
0

보완 제품

Primary Antibodies

AB240636

Anti-Ctip2 antibody [EPR23120-25]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (AB240636)

5
1

Primary Antibodies

AB183032

Anti-TBR1 antibody [EPR8138(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

5
6

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

5
24

Primary Antibodies

AB319166

Anti-TBR2 / Eomes antibody [RM2055]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [RM2055] (AB319166)

0
0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SATB2 protein also known as Special AT-rich Sequence-Binding Protein 2 is a DNA-binding protein with a mass of about 82 kDa. It plays a role in chromatin organization and transcriptional regulation by binding to AT-rich sequences within the nuclear matrix. SATB2 shows high expression levels in the cerebral cortex and the bone where it influences gene expression. It functions by facilitating the assembly of transcriptional machineries enhancing or repressing the transcription of its target genes.

Biological function summary

SATB2 acts as a regulator of multiple developmental processes. It serves as a master control gene in osteoblast differentiation and skeletal development by forming complexes with core binding factors. In the brain SATB2 contributes to the development and maintenance of neuronal networks that are essential for cognitive functions. Its binding to matrix attachment regions aids in the spatial organization of nuclear chromatin impacting gene expression pertinent to development and differentiation.

Pathways

SATB2 operates within the Wnt signaling pathway and the regulation of osteoblast differentiation. It interacts with proteins such as RUNX2 and β-catenin which play roles in bone formation and homeostasis. Within these pathways SATB2 modulates gene networks that influence the expression of structural and signaling proteins important for tissue differentiation and repair particularly in bone and neuronal contexts.

SATB2 has associations with cleft palate and intellectual disabilities conditions often related to alterations in craniofacial and neurological development. It interacts with proteins like SOX9 and TCF7L1 which have roles in developmental processes that when disrupted can lead to such conditions. Understanding SATB2's involvement in these disorders provides insights into potential therapeutic targets for interventions aiming to mitigate developmental abnormalities.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

See full target information SATB2

대체 명칭 보기

KIAA1034, SATB2, DNA-binding protein SATB2, Special AT-rich sequence-binding protein 2

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 8:eabq4736 PubMed36179027

2022

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Ma,Li Pear Wan,Yuwei Li,Chun-Hui He,Ning-Ning Song,Shiping Zhao,Huishan Wang,Yu-Qiang Ding,Bingyu Mao,Nengyin Sheng

eLife 11: PubMed35506651

2022

Generation of vascularized brain organoids to study neurovascular interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yao Sun,Xiang-Chun Ju,Yang Li,Peng-Ming Zeng,Jian Wu,Ying-Ying Zhou,Li-Bing Shen,Jian Dong,Yue-Jun Chen,Zhen-Ge Luo

Cell reports 35:109290 PubMed34192541

2021

The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Yi Ou,Qi Xiao,Xiang-Chun Ju,Peng-Ming Zeng,Jing Huang,Ai-Li Sheng,Zhen-Ge Luo

Nature communications 10:2966 PubMed31273213

2019

Mutations in SMARCB1 and in other Coffin-Siris syndrome genes lead to various brain midline defects.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Filatova,Linda K Rey,Marion B Lechler,Jörg Schaper,Maja Hempel,Renata Posmyk,Krzysztof Szczaluba,Gijs W E Santen,Dagmar Wieczorek,Ulrike A Nuber

Experimental and therapeutic medicine 14:3507-3512 PubMed29042940

2017

Expression and localization of special AT-rich sequence binding protein 2 in murine molar development and the pulp-dentin complex of human healthy teeth and teeth with pulpitis.

Applications

Unspecified application

Species

Unspecified reactive species

Lina He,Huimei Liu,Lei Shi,Shuang Pan,Xu Yang,Lin Zhang,Yumei Niu

Cellular and molecular neurobiology 35:205-16 PubMed25239161

2014

Satb2-independent acquisition of the cholinergic sudomotor phenotype in rodents.

Applications

Unspecified application

Species

Rat

Burkhard Schütz,Martin K-H Schäfer,Markus Gördes,Lee E Eiden,Eberhard Weihe

Tissue engineering. Part A 17:1767-76 PubMed21385070

2011

Roles of SATB2 in osteogenic differentiation and bone regeneration.

Applications

IHC-P

Species

Mouse

Jin Zhang,Qisheng Tu,Rudolf Grosschedl,Min Seok Kim,Terrence Griffin,Hicham Drissi,Pishan Yang,Jake Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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