AB99951

Anti-SBCAD antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal SBCAD antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial aa 150 to C-terminus.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (AB99951)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (AB99951)

ab99951, at 1/100 dilution, staining SBCAD in human fetal colon.

Unknown

Western blot - Anti-SBCAD antibody (AB99951)

All lanes:

Western blot - Anti-SBCAD antibody (ab99951) at 1/500 dilution

All lanes:

Human fetal liver lysate

Predicted band size: 47 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P45954

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.02% Sodium azide

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SBCAD also known as short/branched-chain acyl-CoA dehydrogenase is an enzyme involved in the mitochondrial fatty acid beta-oxidation pathway. It has a molecular mass of approximately 42 kDa. The enzyme functions to catalyze the initial step of the beta-oxidation process specifically targeting short and branched-chain acyl-CoA substrates. SBCAD shows widespread expression notably in tissues with high fatty acid metabolism such as the liver heart and skeletal muscle.

Biological function summary

This enzyme plays a critical role in energy production by facilitating the breakdown of fatty acids to produce acetyl-CoA which subsequently enters the citric acid cycle for ATP generation. SBCAD is not a part of a larger enzyme complex but works in conjunction with other enzymes in the beta-oxidation pathway. Its function is essential for maintaining energy homeostasis particularly during periods when carbohydrates may be less available and the body relies on stored fats.

Pathways

The main involvement of SBCAD is in the fatty acid degradation and energy metabolism pathways. It is closely related to other acyl-CoA dehydrogenases such as medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VLCAD) which also participate in the stepwise oxidation of different acyl-CoA derivatives. Together these enzymes ensure efficient fatty acid breakdown and energy release through the coordinated action of the beta-oxidation cycle leading into the citric acid cycle.

Mutations in the SBCAD gene can lead to short/branched-chain acyl-CoA dehydrogenase deficiency a metabolic disorder characterized by an inability to properly break down certain types of fatty acids. This condition can result in various symptoms including muscle weakness hypoglycemia and metabolic crises often aggravated by fasting or illness. SBCAD's function interlinks with other metabolic enzymes where its deficiency might show overlapping symptoms with disorders involving MCAD and other related enzymes in the beta-oxidation sequence. Understanding SBCAD’s role is important in diagnosing and managing metabolic conditions linked to fatty acid oxidation defects.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Short and branched chain specific acyl-CoA dehydrogenase that catalyzes the proR-proR stereospecific alpha,beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 10832746, PubMed : 11013134, PubMed : 21430231, PubMed : 7698750). Among the different mitochondrial acyl-CoA dehydrogenases, acts specifically on short and branched chain acyl-CoA derivatives such as (S)-2-methylbutyryl-CoA as well as short straight chain acyl-CoAs such as butyryl-CoA (butanoyl-CoA) (PubMed : 10832746, PubMed : 11013134, PubMed : 21430231, PubMed : 7698750). Plays an important role in the metabolism of L-isoleucine by catalyzing the dehydrogenation of 2-methylbutyryl-CoA, one of the steps of the L-isoleucine catabolic pathway (PubMed : 10832746, PubMed : 11013134). Can also act on valproyl-CoA, a metabolite of valproic acid, an antiepileptic drug (PubMed : 8660691).

See full target information Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial

대체 명칭 보기

SBCAD, 2-methyl branched chain acyl-CoA dehydrogenase, 2-methylbutyryl-coenzyme A dehydrogenase, 2-MEBCAD, 2-methylbutyryl-CoA dehydrogenase, ACADSB

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:9550 PubMed39500869

2024

Inhibition of GPX4 enhances CDK4/6 inhibitor and endocrine therapy activity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M T Herrera-Abreu,J Guan,U Khalid,J Ning,M R Costa,J Chan,Q Li,J-P Fortin,W R Wong,P Perampalam,A Biton,W Sandoval,J Vijay,M Hafner,R Cutts,G Wilson,J Frankum,T I Roumeliotis,J Alexander,O Hickman,R Brough,S Haider,J Choudhary,C J Lord,A Swain,C Metcalfe,N C Turner

The Journal of biological chemistry 300:107662 PubMed39128713

2024

Differential utilization of vitamin B-dependent and independent pathways for propionate metabolism across human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Harsha Gouda,Yuanyuan Ji,Sneha Rath,David Watkins,David Rosenblatt,Vamsi Mootha,Jace W Jones,Ruma Banerjee

Cell metabolism 29:1151-1165.e6 PubMed30661928

2019

Loss of BCAA Catabolism during Carcinogenesis Enhances mTORC1 Activity and Promotes Tumor Development and Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Russell E Ericksen,Siew Lan Lim,Eoin McDonnell,Wai Ho Shuen,Maya Vadiveloo,Phillip J White,Zhaobing Ding,Royston Kwok,Philip Lee,George K Radda,Han Chong Toh,Matthew D Hirschey,Weiping Han

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SBCAD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (AB99951)

Western blot - Anti-SBCAD antibody (AB99951)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Reconstitution

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise