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AB221764

Anti-SC5DL antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal SC5DL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SC5D aa 50-100.

대체 명칭 보기

SC5DL, SC5D, Lathosterol oxidase, C-5 sterol desaturase, Delta(7)-sterol 5-desaturase, Delta(7)-sterol C5(6)-desaturase, Lathosterol 5-desaturase, Sterol-C5-desaturase

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SC5DL antibody (AB221764)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SC5DL antibody (AB221764)

PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for SC5DL (green) using ab221764 at 4 μg/ml in ICC/IF.

Western blot - Anti-SC5DL antibody (AB221764)
  • WB

Supplier Data

Western blot - Anti-SC5DL antibody (AB221764)

All lanes:

Western blot - Anti-SC5DL antibody (ab221764) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 35 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SC5D aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O75845

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보완 제품

Primary Antibodies

AB103296

Anti-DHCR7 antibody

Western blot - Anti-DHCR7 antibody (AB103296)

  • 0
  • 0
Primary Antibodies

AB181062

Anti-Seladin 1 antibody [EPR10743]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Seladin 1 antibody [EPR10743] (AB181062)

  • 0
  • 0
Primary Antibodies

AB97448

Anti-IDI1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDI1 antibody (AB97448)

  • 3
  • 1
Primary Antibodies

AB153805

Anti-FDPS/FPS antibody

Western blot - Anti-FDPS/FPS antibody (AB153805)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SC5DL also known as sterol-C5-desaturase or MIG12 is an enzyme important in cholesterol biosynthesis. Weighing approximately 39 kDa SC5DL catalyzes the conversion of lathosterol to 7-dehydrocholesterol by introducing a C5-C6 double bond in the sterol ring. This enzyme predominantly expresses in the liver but is also found in lesser amounts in other tissues such as the brain and skin.
Biological function summary

This enzyme plays a role in the synthesis of cholesterol an essential lipid molecule for membrane structure and function. SC5DL operates within the endoplasmic reticulum often as part of a complex with other enzymes in the cholesterol synthesis pathway. This activity serves not just in producing cholesterol for cell membrane integrity but also generates precursor molecules for steroid hormone production and vitamin D synthesis.

Pathways

SC5DL is an integral part of the mevalonate pathway a critical metabolic pathway for cholesterol synthesis. This pathway involves multiple enzymes and SC5DL works closely with others like HMG-CoA reductase. SC5DL also participates in the Kandutsch-Russell pathway interacting with proteins such as DHCR7 to regulate the flow of sterol intermediates and maintain cholesterol homeostasis.

Alterations in SC5DL activity can impact cholesterol levels and are linked to conditions such as congenital disorders of cholesterol biosynthesis. Mutations or dysfunctions in SC5DL associate with lathosterolosis causing developmental abnormalities due to impaired cholesterol synthesis. Additionally changes in expression of SC5DL and its interaction with proteins like DHCR7 have been studied for their implications in metabolic disorders like Smith-Lemli-Opitz syndrome.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the penultimate step of the biosynthesis of cholesterol, the dehydrogenation of lathosterol into 7-dehydrocholesterol (7-DHC). Cholesterol is the major sterol component in mammalian membranes and a precursor for bile acid and steroid hormone synthesis (PubMed : 10786622, PubMed : 38297129). In addition to its essential role in cholesterol biosynthesis, it also indirectly regulates ferroptosis through the production of 7-DHC. By diverting the spread of damage caused by peroxyl radicals from the phospholipid components to its sterol nucleus, 7-DHC prevents this form of cell death (PubMed : 38297129, PubMed : 38297130).
See full target information SC5D
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 27:110651 PubMed39262789

2024

Knockouts of , , or from cholesterol synthesis reveal pathways modulated by sterol intermediates.

Applications

Unspecified application

Species

Unspecified reactive species

Cene Skubic,Hana Trček,Petra Nassib,Tinkara Kreft,Andrew Walakira,Katka Pohar,Sara Petek,Tadeja Režen,Alojz Ihan,Damjana Rozman

Journal of molecular histology 50:63-73 PubMed30535733

2018

Subcellular localization of sterol biosynthesis enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Katalin Koczok,Channabasavaiah B Gurumurthy,István Balogh,Zeljka Korade,Károly Mirnics
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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