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AB65163

Anti-SCN2A antibody

1

(1 리뷰)

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(3 제품이 사용된 논문 )

Rabbit Polyclonal SCN2A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SCN2A.
1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SCN2A antibody (AB65163)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCN2A antibody (AB65163)

ICC/IF image of ab65163 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65163, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human SCN2A. The exact immunogen used to generate this antibody is proprietary information.

Q99250

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보완 제품

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB65166

Anti-Nav1.6/SCN8A antibody

Western blot - Anti-Nav1.6/SCN8A antibody (AB65166)

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  • 4
Primary Antibodies

AB40801

Anti-DARPP32 antibody [EP720Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (AB40801)

  • 4
  • 11
Primary Antibodies

AB307138

Anti-TAC1 antibody [EPR26567-18-1-3]

BOND RX™ Validated|RabMAb|20ul selling size|Recombinant|Advanced Validation

Immunohistochemistry - Anti-TAC1 antibody [EPR26567-18-1-3] (AB307138)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SCN2A gene encodes for the Nav1.2 protein a voltage-gated sodium channel alpha subunit with a molecular weight of approximately 260 kDa. This protein acts mechanically by allowing sodium ions to flow into neurons upon depolarization making it fundamental for transmitting electrical signals. Nav1.2 is mainly expressed in the central nervous system especially in the brain regions involved in cognitive functions. Besides Nav1.2 SCI 2A is a popular alternate name for this protein.
Biological function summary

The Nav1.2 channel contributes to the generation and propagation of action potentials in neurons. It is part of a larger sodium channel complex that regulates neuronal excitability and synaptic transmission. This channel plays an essential role in maintaining the electrical activity that supports processes like learning and memory. Proper function of Nav1.2 ensures precise neuronal communication which is necessary for normal cognitive and neurological activities.

Pathways

The Nav1.2 channel integrates into the action potential pathway where it participates in the initiation and transmission of nerve impulses. It also intersects with synaptic transmission pathways working alongside proteins like Nav1.1 and Nav1.6 to ensure proper synaptic function. These interactions facilitate an effective communication system necessary for maintaining neural network stability.

Altered Nav1.2 function associates with epilepsy particularly early-onset epileptic encephalopathies. The gene mutations can disrupt normal sodium channel activity leading to increased neuronal excitability and seizure susceptibility. Furthermore variants in SCN2A link to autism spectrum disorders implicating sodium channel deficiencies in abnormal neurodevelopment. The protein interacts with auxiliary proteins like Navβ1 which influences the severity and presentation of these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed : 1325650, PubMed : 17021166, PubMed : 28256214, PubMed : 29844171). Implicated in the regulation of hippocampal replay occurring within sharp wave ripples (SPW-R) important for memory (By similarity).
See full target information SCN2A

대체 명칭 보기

HBA, NAC2, SCN2A1, SCN2A2, SCN2A, Sodium channel protein type 2 subunit alpha, HBSC II, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.2
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Neuron 112:1133-1149.e6 PubMed38290518

2024

Physical and functional convergence of the autism risk genes Scn2a and Ank2 in neocortical pyramidal cell dendrites.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew D Nelson,Amanda M Catalfio,Julie P Gupta,Lia Min,René N Caballero-Florán,Kendall P Dean,Carina C Elvira,Kimberly D Derderian,Henry Kyoung,Atehsa Sahagun,Stephan J Sanders,Kevin J Bender,Paul M Jenkins

Cell reports 36:109483 PubMed34348157

2021

Paradoxical hyperexcitability from Na1.2 sodium channel loss in neocortical pyramidal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Perry W E Spratt,Ryan P D Alexander,Roy Ben-Shalom,Atehsa Sahagun,Henry Kyoung,Caroline M Keeshen,Stephan J Sanders,Kevin J Bender

Journal of cell science 124:1136-44 PubMed21385842

2011

Regulation of mouse embryonic stem cell self-renewal by a Yes-YAP-TEAD2 signaling pathway downstream of LIF.

Applications

Unspecified application

Species

Unspecified reactive species

Christoffer Tamm,Nathalie Böwer,Cecilia Annerén
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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