AB79172

Anti-SCNN1B (phospho T615) antibody

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Rabbit Polyclonal SCNN1B phospho T615 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SCNN1B pT615.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (AB79172)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (AB79172)

Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide.

Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (AB79172)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (AB79172)

Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.

Unknown

Western blot - Anti-SCNN1B (phospho T615) antibody (AB79172)

All lanes:

Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution

Lane 1:

extracts from HeLa cells at 10 µg

Lane 2:

extracts from HeLa cells at 10 µg with immunizing phosphopeptide

Predicted band size: 73 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SCNN1B pT615. The exact immunogen used to generate this antibody is proprietary information.

P51168

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SCNN1B also known as the beta subunit of the epithelial sodium channel (ENaC) is a critical component in sodium ion transport across epithelial cells. SCNN1B has a molecular mass of approximately 92 kDa. It is primarily expressed in epithelial tissues like the kidney lung and colon where it contributes to maintenance of sodium balance and regulation of fluid homeostasis. The protein plays an essential role in controlling sodium absorption which is necessary for various physiological processes.

Biological function summary

SCNN1B is a part of the epithelial sodium channel complex which includes two other subunits: SCNN1A (alpha) and SCNN1G (gamma). Together with these subunits SCNN1B facilitates the selective transport of sodium ions impacting processes such as blood pressure regulation and airway surface liquid homeostasis. The channel formed by these subunits operates under guidance by aldosterone a hormone that influences its assembly and activity.

Pathways

SCNN1B takes part in the Renin-Angiotensin-Aldosterone System (RAAS) and sodium reabsorption pathways. These are vital for maintaining electrolyte balance and blood pressure regulation. SCNN1B interacts closely with proteins such as Nedd4-2 which modulates its activity by influencing channel degradation. This interaction ensures that sodium transport and fluid balance remain tightly regulated.

Abnormalities in SCNN1B function relate to conditions like Liddle syndrome and hypertension. Mutations causing hyperactivity of the channel can lead to excessive sodium reabsorption resulting in these conditions. Furthermore SCNN1B has connections with other proteins like SGK1 which plays a regulatory role in adapting sodium channel activity. Understanding these links is important for targeting these conditions with medical therapies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis (PubMed : 30251954, PubMed : 32729833, PubMed : 7762608, PubMed : 9792722). ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically (PubMed : 24124190). It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys (PubMed : 12107247). Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance (PubMed : 24124190).

See full target information SCNN1B pT615

대체 명칭 보기

Epithelial sodium channel subunit beta, Beta-ENaC, ENaC subunit beta, ENaCB, Epithelial Na(+) channel subunit beta, Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB, SCNN1B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Physiological reports 2: PubMed25347857

2014

Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells.

Applications

Species

Human

Michael J Shimko,Eric J Zaccone,Janet A Thompson,Diane Schwegler-Berry,Michael L Kashon,Jeffrey S Fedan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCNN1B (phospho T615) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (AB79172)

Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (AB79172)

Western blot - Anti-SCNN1B (phospho T615) antibody (AB79172)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise