JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235254

Anti-SCP3 antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal SCP3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication.
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)

Negative control : Immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue sections labeling SCP3 with ab235254 at a 1/100 dilution (30mins at RT), followed by a ready to use anti-Rabbit poly-HRP-IgG secondary (8mins at RT).

Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)

Immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue sections labeling SCP3 with ab235254 at a 1/100 dilution (30mins at RT), followed by a ready to use anti-Rabbit poly-HRP-IgG secondary (8mins at RT).

Counterstained with hematoxylin.

Western blot - Anti-SCP3 antibody (AB235254)
  • WB

Supplier Data

Western blot - Anti-SCP3 antibody (AB235254)

Blocking buffer : 5% BLOTTO.

All lanes:

Western blot - Anti-SCP3 antibody (ab235254) at 5 µg/mL

Lane 1:

Mouse testis whole tissue lysate at 35 µg

Lane 2:

Human testis whole tissue lysate at 35 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 35 µg

Secondary

All lanes:

Goat anti-Rabbit IgG at 1/70000 dilution

Predicted band size: 28 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

대체품

Primary Antibodies

AB97672

Anti-SCP3 antibody [Cor 10G11/7]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

  • 5
  • 10
Primary Antibodies

AB181746

Anti-SCP3 antibody [6F9C5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [6F9C5] (AB181746)

  • 0
  • 1
Primary Antibodies

AB205846

Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7]

BOND RX™ Validated

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (AB205846)

  • 5
  • 1
Primary Antibodies

AB181354

Anti-SCP3 antibody [EPR12695(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SCP3 antibody [EPR12695(B)] (AB181354)

  • 0
  • 0
Primary Antibodies

AB264093

Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (AB264093)

  • 0
  • 0
Primary Antibodies

AB250482

Anti-SCP3 antibody [EPR12695(B)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SCP3 antibody [EPR12695(B)] - BSA and Azide free (AB250482)

  • 0
  • 0

보완 제품

Primary Antibodies

AB13840

Anti-DDX4 / MVH antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (AB13840)

  • 5
  • 22
Primary Antibodies

AB175191

Anti-SCP1 antibody [EP7918]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (AB175191)

  • 4
  • 3
Primary Antibodies

AB192241

Anti-REC8 antibody [EPR16189]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (AB192241)

  • 2
  • 3
Primary Antibodies

AB49602

Anti-Stra8 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (AB49602)

  • 4
  • 10
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
보관 버퍼
Preservative: 0.01% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.
Biological function summary

SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.

Pathways

SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.

SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed : 11311943, PubMed : 22761579). Required for centromere pairing during meiosis in male germ cells (PubMed : 22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed : 10678170). Plays a lesser role in female fertility (PubMed : 10678170, PubMed : 12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed : 22346761).
See full target information Sycp3

대체 명칭 보기

Scp3, Sycp3, Synaptonemal complex protein 3, SCP-3
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 6:eaba7589 PubMed32582856

2020

Self-assembled cGAMP-STINGΔTM signaling complex as a bioinspired platform for cGAMP delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Yanpu He,Celestine Hong,Emily Z Yan,Samantha J Fletcher,Ge Zhu,Mengdi Yang,Yingzhong Li,Xin Sun,Darrell J Irvine,Jiahe Li,Paula T Hammond
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com