AB248114

Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
각 태그의 뜻

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Rabbit Recombinant Monoclonal SCRIBBLE antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

3 이미지

Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114)

This data was developed using ab125080, the same antibody clone in a different buffer formulation.

ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).

Lab

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114)

This data was developed using 125080, the same antibody clone in a different buffer formulation.

Lanes 1-2 : Merged signal (red and green). Green - ab125080 observed at 240 kDa. Red - loading control ab8245 observed at 36 kDa.

ab125080 Anti-SCRIBBLE antibody [EPR4140(2)] was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (<a href='/ko/products/primary-antibodies/scribble-antibody-epr41402-ab125080'>ab125080</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SCRIB (SCRIBBLE) knockout HeLa cell lysate (<a href='/ko/products/cell-lysates/human-scrib-scribble-knockout-hela-cell-lysate-ab257660'>ab257660</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 175 kDa

Observed band size: 240 kDa

false

Unknown

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114)

This data was developed using ab125080, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (<a href='/ko/products/primary-antibodies/scribble-antibody-epr41402-ab125080'>ab125080</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 175 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4140(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab248114 is the carrier-free version of ab125080.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SCRIBBLE also known as SCRIB or Scribble homolog is a protein that mainly functions in cell polarity and adhesion. It has a mass of about 215 kDa. SCRIBBLE interacts with other cellular structures to maintain apical-basal polarity. Researchers observe its expression in several tissue types especially in epithelial tissues where maintaining cell polarity is important. SCRIBBLE acts as a scaffold that links signaling molecules and components of membranes facilitating the organization of molecular assemblies on the plasma membrane.

Biological function summary

SCRIBBLE contributes significantly to cell orientation and tissue architecture. It is a critical component of the Scribble complex which also includes the proteins LGL1 and DLG1. This complex modulates cell junctions and the establishment of cell polarity. SCRIBBLE plays roles in processes such as wound healing and neural growth by modulating cell migration. It also influences epithelial-mesenchymal transition (EMT) a process important in development and cancer metastasis.

Pathways

SCRIBBLE is integral in the regulation of the Hippo signaling pathway. Proper function of this pathway is necessary for controlling organ size and suppressing tumorigenesis. SCRIBBLE interacts with proteins such as YAP and TAZ which act as transcriptional co-activators within the Hippo pathway. Additionally SCRIBBLE is linked to the Wnt signaling pathway where it interacts with Disheveled proteins impacting processes such as cell fate and proliferation.

SCRIBBLE is implicated in cancer progression particularly in breast and colorectal cancers. Disruption in SCRIBBLE's function or expression can lead to loss of cell polarity and increased invasiveness contributing to tumor development. In breast cancer its interaction with the protein LATS2 in the Hippo pathway is important for controlling cell proliferation and apoptosis. Abnormal SCRIBBLE expression is also associated with neural tube defects where proper cell polarity and signaling are required for normal neural development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization (PubMed : 15182672, PubMed : 16344308, PubMed : 16965391, PubMed : 18641685, PubMed : 18716323, PubMed : 19041750, PubMed : 27380321). Via its interaction with CRTAM, required for the late phase polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG and IL22 production (By similarity). Plays a role in cell directional movement, cell orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). Most probably functions in the establishment of apico-basal cell polarity (PubMed : 16344308, PubMed : 19041750). May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium (PubMed : 16965391, PubMed : 19041750). May regulate cell invasion via MAPK-mediated cell migration and adhesion (PubMed : 18641685, PubMed : 18716323). May play a role in exocytosis and in the targeting of synaptic vesicles to synapses (PubMed : 15182672). Functions as an activator of Rac GTPase activity (PubMed : 15182672).

See full target information SCRIB

대체 명칭 보기

CRIB1, KIAA0147, LAP4, SCRB1, VARTUL, SCRIB, Protein scribble homolog, Scribble, hScrib, Protein LAP4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114)

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114)

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise