AB307722

Anti-SCXA antibody [EPR26997-33]

Name: Anti-SCXA antibody [EPR26997-33]
Brand: Abcam Limited
SKU: AB307722
Rating: 5 (1 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SCXA antibody. Suitable for IHC-P and reacts with Rat, Mouse samples.

대체 명칭 보기

BHLHA41, BHLHA48, SCXA, SCXB, SCX, Basic helix-loop-helix transcription factor scleraxis, Class A basic helix-loop-helix protein 41, Class A basic helix-loop-helix protein 48, bHLHa41, bHLHa48

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on adult mouse cerebrum.The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on adult rat cerebrum.The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

Immunohistochemical analysis of paraffin-embedded Rat embryonic 14.5 t tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on tendons of embryonic 14.5 rat.The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

Immunohistochemical analysis of paraffin-embedded Mouse embryonic 14.5 tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on tendons of embryonic 14.5 mouse (PMID : 10477299).The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26997-33

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SCXA also known as Scleraxis A is a transcription factor with a molecular mass of approximately 23 kDa. This protein plays a significant mechanical role in gene expression regulation particularly in connective tissues where it binds to specific DNA sequences. SCXA expression occurs in tendons ligaments and other fibrous tissues where it serves an essential function in development and homeostasis.

Biological function summary

The Scleraxis A transcription factor regulates the differentiation of progenitor cells into tenocytes the cells that form tendons. SCXA operates within a transcriptional complex that directs the expression of genes necessary for tendon extracellular matrix synthesis and organization. The regulation by SCXA ensures the structural integrity and function of tendons and connective tissues critical for movement and force transmission.

Pathways

SCXA participates in key developmental signaling processes including the TGF-beta signaling pathway and the Notch signaling pathway. These pathways influence various biological processes related to cellular differentiation and tissue development. SCXA interacts with other proteins such as Mohawk homeobox (MKX) which also functions in tendon development modulating the cellular responses through these pathways to impact the differentiation and maturation of connective tissue cells.

Aberrant SCXA expression or function links to tendon-related pathologies and fibrotic conditions. Research shows potential connections between SCXA and conditions like tendinopathy characterized by tendon degeneration and impaired healing. SCXA also interacts with proteins such as scleraxis B (SCXB) in the pathological context where disruptions in their activity may lead to changes in tendon structure and mechanics impacting tissue response to stress and injury.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

See full target information SCX

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCXA antibody [EPR26997-33]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] (AB307722)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise