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AB154974

Anti-SDHB antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal SDHB antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SDHB aa 50 to C-terminus.

대체 명칭 보기

SDH, SDH1, SDHB, Iron-sulfur subunit of complex II, Malate dehydrogenase [quinone] iron-sulfur subunit, Ip

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody (AB154974)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody (AB154974)

Immunofluorescence analysis of HeLa cells fixed in MeOH for 5 min labelling SDHB protein using ab154974 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody (AB154974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody (AB154974)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for SDHB protein with ab154974 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody (AB154974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody (AB154974)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue stained for SDHB protein with ab154974 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SDHB aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P21912

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대체품

Primary Antibodies

AB178423

Anti-SDHB antibody [EPR13042(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (AB178423)

  • 5
  • 1
Primary Antibodies

AB151684

Anti-SDHB antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody (AB151684)

  • 0
  • 0
Primary Antibodies

AB198329

HRP Anti-SDHB antibody [EPR10880]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SDHB antibody [EPR10880] (AB198329)

  • 0
  • 0
Primary Antibodies

AB198330

Biotin Anti-SDHB antibody [EPR10880]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-SDHB antibody [EPR10880] (AB198330)

  • 0
  • 0
Primary Antibodies

AB197903

HRP Anti-SDHB antibody [21A11AE7]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SDHB antibody [21A11AE7] (AB197903)

  • 0
  • 0
Primary Antibodies

AB175225

Anti-SDHB antibody [EPR10880]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR10880] (AB175225)

  • 0
  • 0
Primary Antibodies

AB14714

Anti-SDHB antibody [21A11AE7]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (AB14714)

  • 5
  • 12

보완 제품

Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
Primary Antibodies

AB203912

Anti-MTCO1 antibody [EPR19628]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] (AB203912)

  • 0
  • 0
Primary Antibodies

AB203832

Anti-UQCRC2 antibody [EPR13051]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (AB203832)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein SDHB also known as Succinate Dehydrogenase Complex Iron Sulfur Subunit B plays a mechanical role as a component of the succinate dehydrogenase (SDH) enzyme. This subunit has a molecular mass of approximately 30 kDa. SDHB is part of the mitochondrial inner membrane where it is expressed in high levels across various tissues including heart kidney and muscle. It contributes to the electron transport chain by facilitating the oxidation of succinate to fumarate an important step in energy production through the tricarboxylic acid (TCA) cycle.
Biological function summary

SDHB participates in the succinate dehydrogenase complex acting within both the TCA cycle and the electron transport chain. By transferring electrons to coenzyme Q it helps generate an electrochemical gradient important for ATP synthesis. This complex also called Complex II includes other subunits such as SDHA SDHC and SDHD. Their interactions ensure proper function of metabolic processes within mitochondria bridging the gap between foundational energy metabolism and complex cellular processes.

Pathways

SDHB plays an important role in cellular respiration and metabolic cycles. It resides in the TCA cycle and the mitochondrial electron transport chain connecting its function to energy generation pathways. Specifically SDHB relates closely to SDHA and coenzyme Q all working together to facilitate electron transfer and effective mitochondrial energy output. The transfer of electrons through this pathway highlights its essential contribution to maintaining cellular energy homeostasis.

SDHB mutations are associated with conditions such as paraganglioma and pheochromocytoma. These genetic alterations disrupt normal complex II function leading to the accumulation of succinate and succinate-related oncogenesis. In addition to causing tumor development defective SDHB is linked with familial paraganglioma syndromes. Its interaction with proteins such as SDHA and SDHD critical for the succinate dehydrogenase functionality highlights its significant role in maintaining metabolic balance and when defective contributing to disease pathogenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 26925370, PubMed : 27604842). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity).
See full target information SDHB
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS genetics 21:e1011597 PubMed39908322

2025

Plant-specific cochaperone SSR1 affects root elongation by modulating the mitochondrial iron-sulfur cluster assembly machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanjun Feng,Yue Hu,Tao Xie,Huiling Han,Diana Bonea,Lijuan Zeng,Jie Liu,Wenhan Ying,Bona Mu,Yuanyuan Cai,Min Zhang,Yanli Lu,Rongmin Zhao,Xuejun Hua

EBioMedicine 108:105342 PubMed39321499

2024

Cellular and molecular determinants of bacterial burden in leprosy granulomas revealed by single-cell multimodal omics.

Applications

Unspecified application

Species

Unspecified reactive species

Zihao Mi,Zhenzhen Wang,Yi Wang,Xiaotong Xue,Xiaojie Liao,Chuan Wang,Lele Sun,Yingjie Lin,Jianwen Wang,Dianhao Guo,Tingting Liu,Jianjun Liu,Robert L Modlin,Hong Liu,Furen Zhang

Scientific reports 12:20551 PubMed36446868

2022

Fasting increases susceptibility to acute myocardial ischaemia/reperfusion injury through a sirtuin-3 mediated increase in fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew R Hall,Qutuba G Karwi,Sanjeev Kumar,Rachel Dongworth,Dunja Aksentijević,Tariq R Altamimi,Kevin Timothy Fridianto,Kroekkiat Chinda,Sauri Hernandez-Resendiz,Mohammad U Mahmood,Evangelos Michelakis,Chrishan J Ramachandra,Jianhong Ching,Jose M Vicencio,Michael J Shattock,Jean-Paul Kovalik,Derek M Yellon,Gary Lopaschuk,Derek J Hausenloy

Molecular genetics and metabolism 137:342-348 PubMed36335793

2022

GM3 synthase deficiency increases brain glucose metabolism in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sivakama S Bharathi,Bob B Zhang,Eli Paul,Yuxun Zhang,Alexandra V Schmidt,Benjamin Fowler,Yijen Wu,Michael Tiemeyer,Kei-Ichiro Inamori,Jin-Ichi Inokuchi,Eric S Goetzman

Cells 9: PubMed32664214

2020

Inhibition of HDAC1/2 Along with TRAP1 Causes Synthetic Lethality in Glioblastoma Model Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Trang T T Nguyen,Yiru Zhang,Enyuan Shang,Chang Shu,Catarina M Quinzii,Mike-Andrew Westhoff,Georg Karpel-Massler,Markus D Siegelin
제품이 사용된 논문 모두 보기
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Product promise

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