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AB68536

Anti-SDS antibody

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(2 제품이 사용된 논문 )

Mouse Polyclonal SDS antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SDS.
1 이미지
Western blot - Anti-SDS antibody (AB68536)
  • WB

Unknown

Western blot - Anti-SDS antibody (AB68536)

All lanes:

Western blot - Anti-SDS antibody (ab68536) at 1/500 dilution

All lanes:

Human liver lysate at 50 µg

Secondary

All lanes:

Goat anti-mouse (H+L) IgG at 1/2500 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

주요 정보

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SDS.

P20132

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Sodium dodecyl sulfate (SDS) also known as sodium lauryl sulfate is an anionic detergent with a molecular weight of 288.38 g/mol. It disrupts non-covalent bonds in proteins causing them to denature. SDS is widely used for its ability to solubilize proteins by coating them in negative charge ensuring uniform migration during electrophoresis. In laboratory settings SDS is most often expressed in solutions prepared for SDS-PAGE a common technique to separate proteins based on molecular weight.
Biological function summary

Scientists employ sodium dodecyl sulfate in the analysis of protein composition and structure. SDS facilitates the study of protein complexes by breaking apart aggregates into individual polypeptide chains. This function is particularly important in research aiming to determine protein characteristics such as size purity and content. Researchers often pair SDS with reducing agents to further break disulfide bonds in proteins offering clearer insights into the proteins' linear arrangement.

Pathways

The use of sodium dodecyl sulfate is important for revealing information underpinned by protein characterization and separation protocols. In processes like the MAPK/ERK signaling pathway understanding individual protein constituents is important. Additionally related proteins such as kinases and phosphatases have their activities scrutinized through SDS-PAGE analysis enabling better assessment of their roles in signaling pathways.

The accuracy of sodium dodecyl sulfate in protein study helps in understanding conditions such as Alzheimer's disease and some cancers. Scientists analyze variations in protein expression and aggregation considering proteins like amyloid-beta and oncogenes in these disorders. SDS makes it possible to detect these alterations early on which helps in advancing diagnostic and therapeutic strategies against such conditions.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Catalyzes the pyridoxal-phosphate-dependent dehydrative deamination of L-threonine and L-serine to ammonia and alpha-ketobutyrate and pyruvate, respectively.
See full target information SDS

대체 명칭 보기

SDH, SDS, L-serine dehydratase/L-threonine deaminase, Hepatic serine dehydratase, L-serine deaminase, L-threonine dehydratase, hSDH, TDH

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 29:3331-3348.e7 PubMed31801093

2019

Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin D Stein,Diego Calzolari,Kristina Hellberg,Ying S Hu,Lin He,Chien-Min Hung,Erin Q Toyama,Debbie S Ross,Björn F Lillemeier,Lewis C Cantley,John R Yates,Reuben J Shaw

Amino acids 39:145-53 PubMed19921396

2009

The effects of a low protein diet on amino acids and enzymes in the serine synthesis pathway in mice.

Applications

WB

Species

Mouse

Jordan E Antflick,Glen B Baker,David Richard Hampson
제품이 사용된 논문 모두 보기

Product promise

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