AB241585

Anti-SEC22B antibody

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Rabbit Polyclonal SEC22B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SEC22B aa 100-150.

대체 명칭 보기

SEC22L1, SEC22B, Vesicle-trafficking protein SEC22b, ER-Golgi SNARE of 24 kDa, SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, ERS-24, ERS24

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Supplier Data

Immunoprecipitation - Anti-SEC22B antibody (AB241585)

SEC22B was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241585 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241585 at 1 μg/ml.

Lane 1 : ab241585 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in RIPA buffer.

All lanes:

Immunoprecipitation - Anti-SEC22B antibody (ab241585)

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-SEC22B antibody (AB241585)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-SEC22B antibody (ab241585) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 25 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SEC22B aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

O75396

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to SEC22B immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SEC22B also known as SEC22 homolog B is a membrane protein involved in vesicular trafficking within cells. It plays an important role in the transport between the endoplasmic reticulum (ER) and the Golgi apparatus. SEC22B belongs to the SNARE (soluble NSF attachment protein receptor) family with a molecular mass of approximately 29 kDa. It is expressed in various tissues with prominent presence in the immune system and neuronal tissues. The protein participates in the assembly of vesicle transport machinery important for proper cellular function and compartmentalization.

Biological function summary

SEC22B contributes to the fusion of transport vesicles with target membranes. It acts as part of a SNARE complex facilitating vesicle docking and subsequent membrane fusion through protein-protein interaction. In immune cells SEC22B is essential for antigen presentation. It regulates the trafficking of peptide-MHC complexes to the cell surface essential for adaptive immune response. In neurons the protein ensures efficient neurotransmitter release by managing vesicle cycling and synaptic vesicle fusion.

Pathways

SEC22B integrates into the vesicular transport and secretory pathways. It operates in the ER-Golgi transport route critical in maintaining cellular homeostasis and protein processing. The function of SEC22B involves interaction with other SNARE proteins like VAMP7 and SNAP23 forming SNARE complexes that drive membrane fusion events. This protein also influences the Golgi-to-ER retrograde transport contributing to the protein sorting pathway that recycles specific proteins back to the ER.

SEC22B links closely to immune-related conditions and neurodegenerative diseases. Disruption in SEC22B function can impact antigen presentation and immune system efficiency potentially contributing to autoimmune disorders. In neurological contexts altered SEC22B activity may interfere with neurotransmitter release possibly playing a role in conditions such as Alzheimer's disease. Connections with proteins like VAMP7 underline the importance of SEC22B in maintaining cellular integrity across different systems.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.

See full target information SEC22B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 24:102700 PubMed34235408

2021

Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Shadrach,Wesley M Stansberry,Allison M Milen,Rachel E Ives,Elizabeth A Fogarty,Anthony Antonellis,Brian A Pierchala

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC22B antibody

Immunoprecipitation - Anti-SEC22B antibody (AB241585)

Western blot - Anti-SEC22B antibody (AB241585)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise