AB15576

Anti-SEC61B antibody

Name: Anti-SEC61B antibody
Brand: Abcam Limited
SKU: AB15576
Rating: 4 (1 reviews)

(1 리뷰)

(5 제품이 사용된 논문 )

Rabbit Polyclonal SEC61B antibody. Suitable for ICC/IF and reacts with Dog, Mouse, Rat, Human, Pig samples. Cited in 5 publications.

대체 명칭 보기

Protein transport protein Sec61 subunit beta, SEC61B

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB15576)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB15576)

Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (ab15576) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Dog, Human, Pig

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Ab15576 detects human, dog, pig, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purity

Whole antiserum

보관 버퍼

Preservative: 0.05% Sodium azide

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SEC61B also known as Sec61 beta subunit or Protein Transport Protein Sec61 subunit beta is a component of the Sec61 complex. It has a mass of approximately 9 kDa. SEC61B is expressed broadly in various tissues but it is found most abundantly in the endoplasmic reticulum (ER) membrane. This protein functions mechanically as a part of the protein translocation machinery that guides nascent polypeptides into or across the ER membrane.

Biological function summary

SEC61B assists in the regulation and positioning of the Sec61 translocon complex which includes alpha and gamma subunits. This protein is not a gatekeeper of the channel but rather stabilizes the complex and influences co-translational and post-translational translocation of proteins. It plays roles in maintaining ER homeostasis and facilitating the integration of newly synthesized proteins into the ER membrane affecting various cellular processes such as secretion and membrane biogenesis.

Pathways

SEC61B operates in the protein translocation pathways within the ER membrane. It is integral to the secretory pathway that moves proteins from the cytoplasm into the ER lumen or membrane. Additionally SEC61B interacts with proteins like ribosome-bound signal recognition particle (SRP) to facilitate accurate protein targeting and translocation. It associates with other ER resident proteins to manage polypeptide folding and processing.

SEC61B has connections to conditions that involve protein misfolding and improperly handled ER stress such as cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis misfolded proteins can trigger ER stress and cause adaptive responses. The involvement of proteins like the SEC61 complex in ameliorating ER stress highlights potential therapeutic targets. Therefore understanding SEC61B function and regulation may provide insights into therapeutic intervention for ER-stress-related disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER) (PubMed : 12475939). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides (PubMed : 12475939). The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins : it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex (PubMed : 32820719, PubMed : 36261522). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (PubMed : 19121997).

See full target information SEC61B

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 43:113992 PubMed38536815

2024

OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Styliani Panagiotou,Kia Wee Tan,Phuoc My Nguyen,Andreas Müller,Affiong Ika Oqua,Alejandra Tomas,Anna Wendt,Lena Eliasson,Anders Tengholm,Michele Solimena,Olof Idevall-Hagren

Basic research in cardiology 116:63 PubMed34713358

2021

Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimir Bogdanov,Andrew M Soltisz,Nicolae Moise,Galina Sakuta,Benjamin Hernandez Orengo,Paul M L Janssen,Seth H Weinberg,Jonathan P Davis,Rengasayee Veeraraghavan,Sandor Györke

Cell research 31:141-156 PubMed32989223

2020

COPII mitigates ER stress by promoting formation of ER whorls.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Xu,Wanqing Du,Qin Zou,Yuting Wang,Xin Zhang,Xudong Xing,Ying Li,Dachuan Zhang,Huimin Wang,Wenhao Zhang,Xinyao Hu,Xin Liu,Xiaoling Liu,Shaojin Zhang,Jinqiang Yu,Jianhuo Fang,Fajin Li,Ying Zhou,Tieqiang Yue,Na Mi,Haiteng Deng,Peng Zou,Xiaowei Chen,Xuerui Yang,Li Yu

The Journal of cell biology 216:1301-1320 PubMed28404643

2017

The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhao Liu,Yang Li,Xin Wang,Ruxiao Xing,Kai Liu,Qiwen Gan,Changyong Tang,Zhiyang Gao,Youli Jian,Shouqing Luo,Weixiang Guo,Chonglin Yang

Nature 535:164-8 PubMed27383988

2016

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zhang,Jonathan J Miner,Matthew J Gorman,Keiko Rausch,Holly Ramage,James P White,Adam Zuiani,Ping Zhang,Estefania Fernandez,Qiang Zhang,Kimberly A Dowd,Theodore C Pierson,Sara Cherry,Michael S Diamond

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC61B antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB15576)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise