AB244335

Anti-SEC62 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal SEC62 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEC62 aa 250 to C-terminus.

대체 명칭 보기

TLOC1, SEC62, Translocation protein SEC62, Translocation protein 1, TP-1, hTP-1

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SEC62 antibody (AB244335)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEC62 antibody (AB244335)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling SEC62 using ab244335 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for SEC62 with ab244335 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for SEC62 with ab244335 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for SEC62 with ab244335 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Formalin-fixed, paraffin-embedded human prostate tissue stained for SEC62 with ab244335 at a 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-SEC62 antibody (AB244335)

All lanes:

Western blot - Anti-SEC62 antibody (ab244335) at 0.4 µg/mL

All lanes:

A431 (Human epidermoid carcinoma cell line) cell lysate

Predicted band size: 46 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SEC62 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99442

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SEC62 also known as translocation protein SEC62 is an essential component of the protein translocation machinery in cells. It is part of the Sec61 complex and helps with the translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane. SEC62 has a molecular mass of approximately 24 kilodaltons. It is expressed widely in tissues with higher expression levels observed in the pancreas and liver which are essential for protein synthesis and secretion.

Biological function summary

Beyond its role in the ER SEC62 contributes to the maintenance of calcium homeostasis and cellular stress responses. It forms a part of the SEC63 complex working alongside SEC63 and SEC61 to facilitate protein folding and assembly. This protein also plays a role in the response to ER stress where it assists in relieving the burden by managing calcium leakage across the ER membrane which is important for maintaining cell health under stress conditions.

Pathways

SEC62 plays a significant role in the signal recognition particle (SRP) pathway and the unfolded protein response (UPR). It interacts with other proteins in these pathways including SEC63 and BIP to regulate protein processing and transport. The SEC61 complex which includes SEC62 integrates signals for targeting nascent proteins to the ER membrane and aids in managing misfolded protein accumulation through UPR therefore ensuring cellular homeostasis.

SEC62 has been linked to various types of cancer including prostate and thyroid cancer where its overexpression correlates with tumor progression and metastasis. It interacts with proteins like BIP in cancer cells contributing to the ER stress response that supports cancer cell survival. Additionally alterations in SEC62 are associated with congenital disorders like congenital disorders of glycosylation (CDG) where dysfunctional protein translocation and glycosylation processes impact cellular function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mediates post-translational transport of precursor polypeptides across endoplasmic reticulum (ER). Proposed to act as a targeting receptor for small presecretory proteins containing short and apolar signal peptides. Targets and properly positions newly synthesized presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen.

See full target information SEC62

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal for immunotherapy of cancer 12: PubMed38589248

2024

PD-1 downregulation enhances CAR-T cell antitumor efficiency by preserving a cell memory phenotype and reducing exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Wanyan Ouyang,Shi-Wei Jin,Nan Xu,Wei-Yang Liu,Han Zhao,Liuqingqing Zhang,Liqing Kang,Yi Tao,Yuanfang Liu,Yan Wang,Jin Wang,Feng Liu,Lei Yu,Zhiqiang Liu,Jian-Qing Mi

Cell death & disease 14:83 PubMed36739418

2023

VHL-HIF-2α axis-induced SEMA6A upregulation stabilized β-catenin to drive clear cell renal cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ji,Yuxin Xu,Mengru Xie,Xingbei He,Dexu Ren,Teng Qiu,Wenwen Liu,Zefeng Chen,Wen Shi,Zhen Zhang,Xiujun Wang,Weiling Wang,Jinming Ma,Qilan Qian,Aixin Jing,Xinhui Ma,Jingting Qin,Yuanyuan Ding,Ting Geng,Jiayan Yang,Zhichao Sun,Wei Liu,Shunfang Liu,Bin Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC62 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC62 antibody (AB244335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC62 antibody (AB244335)

Western blot - Anti-SEC62 antibody (AB244335)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise