Anti-SEC63 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-SEC63 antibody (AB240567)

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Lane 1 : ab240567 used for IP at 6 μg per reaction.
Lane 2 : Control IgG.

For WB ab240567 was used at 0.4 μg/ml.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SEC63 antibody (ab240567)

Predicted band size: 88 kDa

false

• WB

Supplier Data

Western blot - Anti-SEC63 antibody (AB240567)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-SEC63 antibody (ab240567) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 88 kDa

false

Exposure time: 30s