AB244497

Anti-SEC63 antibody

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Rabbit Polyclonal SEC63 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEC63 aa 50-200.

대체 명칭 보기

DNAJC23, SEC63L, SEC63, Translocation protein SEC63 homolog, DnaJ homolog subfamily C member 23

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-SEC63 antibody (AB244497)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEC63 antibody (AB244497)

PFA-fixed, Triton X-100 permeabilized Hep G2 cells stained for SEC63 (green) using ab244497 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human testis tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human skeletal muscle tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis. No positivity observed.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human pancreas tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Paraffin-embedded human salivary gland tissue stained for SEC63 using ab244497 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-SEC63 antibody (AB244497)

All lanes:

Western blot - Anti-SEC63 antibody (ab244497) at 0.4 µg/mL

All lanes:

SK-MEL-30 (human metastatic malignant melanoma cell line) cell lysate

Predicted band size: 88 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SEC63 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UGP8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SEC63 protein also known as NNF1 plays a critical role in the process of protein translocation across the endoplasmic reticulum (ER) membrane. It has a molecular mass of approximately 80 kDa. SEC63 is widely expressed in various tissues with notable expression in the ER where it functions as part of the translocon complex facilitating the proper insertion and folding of nascent polypeptides into the ER. This protein contains several distinct domains that contribute to its function in protein trafficking and biogenesis within the cell.

Biological function summary

SEC63 participates in cooperation with other components of the Sec61 translocon complex to assist in the translocation of polypeptides from the cytosol into the ER. It is essential for the proper assembly of membrane and secretory proteins directly impacting protein maturation. SEC63 interacts with proteins such as SEC62 and BIP which further stabilize the translocon and assist in the unfolding and refolding of polypeptides. Through this complex SEC63 plays an integral role in maintaining ER homeostasis.

Pathways

SEC63 is an integral component of the protein secretion pathway and the ER-associated degradation (ERAD) pathway. In the protein secretion pathway SEC63 cooperates with proteins like SEC61 to facilitate the proper folding and assembly of proteins destined for secretion. In the ERAD pathway SEC63 aids in recognizing and targeting misfolded proteins for degradation thereby preventing accumulation of defective proteins. By participating in these pathways SEC63 ensures the fidelity of protein quality control and homeostasis within the cellular environment.

Mutations in SEC63 have been linked to polycystic liver disease (PCLD) and can also influence processes related to cancer development. In PCLD the SEC63 protein's impaired function leads to defective protein processing contributing to cyst formation in liver tissue. SEC63's involvement in cancer relates to its role in maintaining protein quality control where disruption can result in an environment conducive to tumor growth. The interaction between SEC63 and proteins such as polycystin-1 and polycystin-2 highlights its importance in these pathological conditions as these proteins are similarly implicated in such diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER) (PubMed : 22375059, PubMed : 29719251). Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen (PubMed : 29719251). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity).

See full target information SEC63

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of Cancer 15:5786-5809 PubMed39308676

2024

Prognostic and Immunological Role of Cuproptosis-Related Gene MTF1 in Pan-Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Sifen Wang,Hailin Tang,Renchun Lai,Qiaoting Cai,Yaorong Su,Hao Wu,Yongwen Huang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC63 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC63 antibody (AB244497)

Western blot - Anti-SEC63 antibody (AB244497)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise