Anti-Securin antibody [EPR3240] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Securin antibody [EPR3240] - BSA and Azide free (AB239892)

ab79546 at 1/100 dilution staining Securin in paraffin-embedded human testis tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, BSA, glycerol, and sodium azide (ab79546).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Securin antibody [EPR3240] - BSA and Azide free (AB239892)

Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab79546 (red) or a rabbit IgG (negative) (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79546).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Securin antibody [EPR3240] - BSA and Azide free (AB239892)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling securin with ab79546 at 1/250 dilution. The cells were permeabilised with 0.1% Triton X-100. Anti-rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79546).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Securin antibody [EPR3240] - BSA and Azide free (AB239892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Securin with purified ab79546 at 1/200 dilution (1.19 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79546)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Securin antibody [EPR3240] - BSA and Azide free (AB239892)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Securin with purified ab79546 at 1/50 dilution (4.74 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79546)

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Securin antibody [EPR3240] - BSA and Azide free (AB239892)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Securin with purified ab79546 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79546)

• WB

Lab

Western blot - Anti-Securin antibody [EPR3240] - BSA and Azide free (AB239892)

This data was developed using ab79546, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

In Western blot, Anti-6X His tag^® antibody [EPR20547] - ChIP Grade (ab213204), at 1/5000 dilution.

All lanes:

Western blot - Anti-Securin antibody [EPR3240] (<a href='/ko/products/primary-antibodies/securin-antibody-epr3240-ab79546'>ab79546</a>) at 1/10000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) + OE- empty whole cell lysate

Lane 2:

293T + OE-Securin whole cell lysate

Lane 3:

293T + OE-Securin-2 whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 28 kDa,36 kDa

false

Exposure time: 3s