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AB235840

Anti-SEH1L antibody [EPR20851] - BSA and Azide free

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Rabbit Recombinant Monoclonal SEH1L antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication.

대체 명칭 보기

SEC13L, SEH1, SEH1L, Nucleoporin SEH1, GATOR2 complex protein SEH1, Nup107-160 subcomplex subunit SEH1, SEC13-like protein

2 이미지
Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)
  • IP

Supplier Data

Immunoprecipitation - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)

SEH1L was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate with ab218531 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218531 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1 : HEK-293T whole cell lysate 10 μg (Input).
Lane 2 : ab218531 IP in HEK-293T whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab218531 in HEK-293T whole cell lysate (-).
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 28199315).

According to the data of WB1, the band above 250 kDa could be protein aggregates containing SEH1L.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

All lanes:

Immunoprecipitation - Anti-SEH1L antibody [EPR20851] (<a href='/ko/products/primary-antibodies/seh1l-antibody-epr20851-ab218531'>ab218531</a>)

Predicted band size: 40 kDa

false

Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)
  • WB

Supplier Data

Western blot - Anti-SEH1L antibody [EPR20851] - BSA and Azide free (AB235840)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID : 28199315). The band above 250 kDa was also decreased in the SEH1L knockdown lysate. This suggests that the band contains SEH1L, possibly in protein aggregates.

The cell lysates were kindly provided by our collaborator Dr. Liang Zhang, Xiamen University.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218531).

All lanes:

Western blot - Anti-SEH1L antibody [EPR20851] (<a href='/ko/products/primary-antibodies/seh1l-antibody-epr20851-ab218531'>ab218531</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

HEK-293T (human embryonic kidney epithelial cell) transfected with shSEH1L vector, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

false

Exposure time: 3min

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20851

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB213181

Anti-DEPDC5 antibody [EPR20497-23]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (AB213181)

  • 5
  • 2
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB202274

Anti-MIOS antibody

Immunoprecipitation - Anti-MIOS antibody (AB202274)

  • 0
  • 0
Primary Antibodies

AB168824

Anti-SEC13L1 antibody

Immunoprecipitation - Anti-SEC13L1 antibody (AB168824)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

ab235840 is the carrier-free version of ab218531.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SEH1L also known as SEH1 Nucleoporin is a protein that plays a mechanical role in the nuclear pore complex (NPC) which is involved in the transport of molecules between the nucleus and cytoplasm. It has a molecular mass of approximately 40 kDa. SEH1L is expressed in various tissues with notable expression in the liver kidney and spleen. In the cellular context it associates with other nucleoporins to form the NPC that spans the nuclear envelope facilitating the exchange of macromolecules.
Biological function summary

SEH1L contributes to the structural framework of the nuclear pore complex by being a part of the Nup107-160 subcomplex. This subcomplex is vital for maintaining NPC assembly and function. In addition to its structural role SEH1L influences the timing of mitotic spindle assembly and chromosome segregation. The protein is also implicated in the regulation of the cell cycle showcasing its importance in normal cellular operations.

Pathways

SEH1L has involvement in the mTOR signaling pathway and the cell cycle control pathway. Within the mTOR pathway it works in coordination with proteins such as Raptor and mTORC1 linking nutrient availability to cell growth. In the cell cycle control pathway SEH1L impacts transitions between different phases by interacting with cyclin-dependent kinases and other regulatory proteins. These interactions highlight its role in ensuring proper cell cycle progression and preventing aberrant cell division.

SEH1L is associated with cancer and developmental disorders. In cancer alterations in SEH1L and its related proteins like mTOR can lead to uncontrolled cellular proliferation and tumor growth. Developmental disorders related to faulty NPC components including SEH1L affect nucleocytoplasmic transport which may result in developmental delays and congenital anomalies. Understanding the function and regulation of SEH1L in these contexts offers potential insights for therapeutic strategies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC (PubMed : 15146057, PubMed : 17363900). The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore (PubMed : 15146057, PubMed : 17363900).. As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway (PubMed : 23723238, PubMed : 25457612, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex (PubMed : 23723238, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1 (PubMed : 36528027). In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation (PubMed : 36528027). In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex (PubMed : 25457612, PubMed : 26972053, PubMed : 27487210). Within the GATOR2 complex, SEC13 and SEH1L are required to stabilize the complex (PubMed : 35831510).
See full target information SEH1L
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular carcinogenesis 62:1935-1946 PubMed37642311

2023

Downregulation of cellular retinoic acid binding protein 1 fosters epithelial-mesenchymal transition in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chiung Hsu,Wen-Chien Huang,Chi-Yu Kuo,Ying-Syuan Li,Shih-Ping Cheng
제품이 사용된 논문 모두 보기
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Product promise

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